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| Lytic Effect of Environmental Factors on Algae-lysing Bacteria Strain S7 by Fluorescence Spectroscopy |
| WANG Jin-Xia, LUO Gu-Yuan, XU Xiao-Yi, YE Jiang-Yu |
| (Key Laboratory of the Three Gorges Reservoir Region’s Ecoenvironment, Ministry of Education, Chongqing University, Chongqing 400045, China) |
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Abstract Anabaena flos-aquae were scanned in vivo by fluorescence spectrum scanning to acquire characteristic fluorescence wavelength of phycocyanin and build a linear relationship between Anabaena flosaquae biomass and the phycocyanin characteristic fluorescence intensity. With the change of cyanobacteria growth conditions, the lytic effects of varying environmental factors (temperature, illumination and pH) to the algaelysing bacterial strain S7 were studied. The results show that utilizing fluorescence spectrum for detecting the phycocyanin fluorescence intensity and reflecting anabaena flosaquae biomass are simple, fast, accurate and feasible. Environmental factors affect the lytic effect of algaelysing bacterial strain S7. Levels of lytic effects are 30 °C>35 °C>40 °C>25 °C, dark＞light cycle＞full light, pH 5 >pH 10>pH 6>pH 9>pH 8>pH 7. For utilizing algicidal bacteria to control water bloom, exploring optimal environmental conditions for restraining the growth of those bacteria is of practical significance.
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Received: 13 October 2011
Published: 28 May 2012
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