组织工程与重建外科杂志 ›› 2022, Vol. 18 ›› Issue (2): 163-.

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3D打印“生物活性支架”过程中细胞损伤的研究进展

  

  • 出版日期:2022-04-01 发布日期:2022-06-22

Research progress of cell damage in the process of 3D printing of “bioactive scaffolds” 

  • Online:2022-04-01 Published:2022-06-22

摘要: 近年来,3D打印“生物活性支架”已成为研究的热点。虽然3D打印生物活性支架取得了巨大的进步,但在生物活性支架打印过程中,多种因素会导致细胞损伤,甚至细胞死亡,主要包括以下几种原因:①不同的打印方式产生的细胞损伤方式及损伤的程度各不相同,如热损伤、挤压损伤、剪切和拉伸应力损伤等;②接收基板是否有涂层,以及涂层的厚度和原材料种类等。接收基板的涂层可以起到细胞培养液和碰撞缓冲的双重作用,会对细胞损伤产生一定程度的影响,细胞损伤的程度直接影响了3D打印生物活性支架的生物活性。本文对生物活性支架打印过程中的细胞损伤的研究进展进行综述。

关键词: 3D打印,  支架,  细胞损伤

Abstract:  In recent years, 3D printing of "bioactive scaffolds" has become a research hotspot. Although great progress has been made in 3D printing of bioactive scaffolds, in the printing process of bioactive scaffolds, many factors will lead to cell damage or even cell death, mainly including the following reasons: 1) Different printing methods can produce different types of cell damage and damage degrees, such as heat damage, extrusion damage, shear damage and tensile stress damage, etc. 2) Whether there is a coating on the receiving substrate, the thickness of the coating and the type of raw materials, etc. The coating on the receiving substrate can play a dual role of cell culture medium and collision buffer, both of which will have a certain degree of influence on cell damage, and the degree of cell damage directly affects the biological activity of the 3D-printed bioactive scaffold. In this paper, the cell damage in the printing process of bioactive scaffolds was reviewed based on the latest research progress.

Key words: 3D printing,  Scaffolds,  Cell damage