In large-scale analytical instrument teaching practice, the main scene graph is that many students stand around the only one machine and listen to the teacher’s speaking in a narrow room. In order to promote the teaching quality, enhance student’s practice ability, innovation ability and collaboration ability, we tried to establish a new pedagogics which is a closed ring of theory explanation—microlecture—accurate in-depth—self test assessment—innovation boosting. The pedagogics has been realized through a teaching management web site, and thus we groped a valid instrument teaching practice with supervisor state. We summarized the advantages of this supervisor state in three aspects: 1. The key points of the experiment can be popularized; 2. Large-scale analytical instrument teaching practice can be carried out effectively; 3. It promotes students’ learning autonomy, learning desire, innovation ability. 4. It provides full process data support for a fare judgment of the student's score.