Objective To screen and identify the genes associated with sensitivity of neoadjuvant therapy for the patients with colorectal cancer through high-throughput transcriptome sequence technology and bioinformatics tools by analysis of the genes which expressed differently. Methods RNA was extracted from the tumor tissue of 22 patients with co-lorectal cancer who received standard neoadjuvant therapy before operation. Nine cases were divided as sensitive group and 11 cases as non-sensitive group based on tumor regression grading. Strand libraries were constructed for high-throughput sequencing. The genes filtered the false discovery rate(<0.05) were mapped to the reference genome, and assembled. The obtained transcripts were classified and annotated and the characteristics of gene were analyzed. Results The gene database associated with neoadjuvant therapy for colorectal cancer was built preliminarily. From 22 patients with colorectal cancer tissues, 89 genes were found up-regulated significantly and 112 genes down-regulated. Conclusions The genes related to the sensitivity of colorectal cancer neoadjuvant therapy were identified in this study using high-throughput transcriptome sequence.
CHEN Xianze, CHENG Xi, WU Haoxuan, ZHANG Tao, JI Xiaopin, ZHAO Ren
. Preliminary research on colorectal cancer neoadjuvant therapy sensitivity through high-throughput transcriptome sequence[J]. Journal of Surgery Concepts & Practice, 2018
, 23(01)
: 67
-71
.
DOI: 10.16139/j.1007-9610.2018.01.015
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