Objective To study myopectineal orifice anatomy of the patients with inguinal groin hernia using three-dimensional imaging technology after reconstructing of myopectineal orifice. Methods Preoperative CT images of 90 patients with inguinal hernia including 79 cases with indirect, 9 cases with direct and 2 cases with femoral were collected in our hospital from March 2019 to December 2019. The data of CT imaging were imported into software for three-dimensional reconstruction. The data of myopectineal orifice anatomy after reconstruction were measured and compared after grouping of age, gender and body mass index. Results The mean total width of myopectineal orifice was(7.67±0.75) cm, length (7.34±0.38) cm, length of upper edge(5.79±0.79) cm, length of lower edge(6.57±0.50) cm, and the upper lower angle (120.10±9.36) °. Difference in total width, length, and upper edge length was statistically significant between male group and female group (P<0.05). There was difference in upper edge length between adult group and old group (P<0.05) and in upper lower angle between body mass index low group and high group (P<0.05). Conclusions Three-dimensional CT ima-ging technology could be feasible to measure the morphology of myopectineal orifice. The data of myopectineal orifice collected in this study would be used to direct prosthetic mesh of inguinal hernia according to different age, gender and body mass index.