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上海交通大学学报(农业科学版)  2016, Vol. 34 Issue (1): 11-15    DOI: 10.3969/J.ISSN.1671-9964.2016.01.003
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牛磺酸对昆明小鼠早期胚胎体外发育和着床的影响
范晶晶1,2(), 郑秋闿3
1.潍坊学院 生物与农业工程学院, 山东 潍坊 261061
2.山东省高校生物化学与分子生物学重点实验室(潍坊学院), 山东 潍坊 261061
3.潍坊学院化学化工与环境工程学院, 山东 潍坊261061
Effectof Taurine on Development and Implantation of Kunming Mouse Early Embryos
Jing-jing FAN1,2(), Qiu-kai ZHENG3
1. Biological and Agricultural Engineering College ofWeifang University, Weifang 261061, China
2. Key Laboratory of Biochemistry & Molecular Biology in Universities of Shandong, Weifang University, Weifang 261061 China
3. College of Chemistry and Chemical Engineering, Weifang University, Weifang 261061, China
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摘要 

本研究主要探讨了牛磺酸对昆明(KM)小鼠体外胚胎发育及对胚胎着床的作用。在mKSOM(modified potassium simplex optimized medium)胚胎体外培养液中分别添加不同浓度牛磺酸, 培养24、72 h后分别统计4-细胞率、囊胚率、囊胚总细胞数; 采用子宫角注射的方法, 研究牛磺酸转运抑制剂β-丙氨酸对孕D9胚胎着床数的影响。结果表明, 添加10 mmol/L牛磺酸培养液组的胚胎4-细胞率高于对照组(P<0.05), 囊胚率与囊胚总细胞数明显高于对照组(P<0.01)。子宫角注射β-丙氨酸(20 μg/mL)显著降低D9着床的胚胎数。本研究初步明确了牛磺酸对小鼠体外胚胎发育及对胚胎着床的影响, 为进一步研究胚胎发育和着床提供参考。
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范晶晶
郑秋闿
关键词 ： 牛磺酸,  胚胎发育,  着床    
Abstract：

The effects of taurine on development and implantation of Kunming mouse early embryos were investigated.The embryos were cultivated in modified potassium simplex optimized medium(mKSOM)with different concentration of taurine.The percentage of 4-cell, blastocysts and average total cell number of blastocysts were calculated after 24 h and 72 h.Cornua uteri injection technique was used to investigate the effects of β-alaline(β-ala, taurine transport inhibitor)on embryo implantation.The results showed that there were significant differences between 10 mmol/L taurine treatment and control in the percentage of 4-cell(P<0.05), blastocysts and total cell number of blastocysts(P<0.01).Meanwhile, injection of β-ala(20 μg/mL)into uterine horn significantly reduced the number of implanted embryos on D9.This study explicated the effects of taurine on the development and implantation of early Kunming mouse embryos, providing a useful reference for further uncovering of mechanism of embryo development and implantation.
Key words： taurine    embryo development    implantation
收稿日期: 2015-02-01      出版日期: 2016-03-08
基金资助:山东省自然科学基金项目(ZR2013BL015);潍坊市科学技术发展计划(20121307);潍坊学院博士基金项目(2010BS04)
作者简介:

作者简介: 范晶晶(1981-), 女, 博士, 讲师, 研究方向:动物生殖与发育研究, E-mail:fanjingjing2008@163.com
引用本文:   
范晶晶, 郑秋闿. 牛磺酸对昆明小鼠早期胚胎体外发育和着床的影响[J]. 上海交通大学学报(农业科学版), 2016, 34(1): 11-15.
Jing-jing FAN, Qiu-kai ZHENG. Effectof Taurine on Development and Implantation of Kunming Mouse Early Embryos. Journal of Shanghai Jiaotong University (Agricultural Sciences), 2016, 34(1): 11-15.
链接本文:  
http://www.qk.sjtu.edu.cn/jsjtua/CN/10.3969/J.ISSN.1671-9964.2016.01.003      或      http://www.qk.sjtu.edu.cn/jsjtua/CN/Y2016/V34/I1/11
Fig.1  牛磺酸对小鼠胚胎体外培养囊胚率的影响
牛磺酸/
(mmol·L-1)
Tau		 β-丙氨酸/
(mmol·L-1)
β-ala		 2-细胞胚胎数
No. of 2-细胞		 胚胎数 No.of embryos 囊胚细胞数
No. of cell
(Mean±SE)		 囊胚细胞数 范围
Range
发育至4细胞个数(比例)
No.
of 4-cell(ratio)		 发育至囊胚个数
(比例)No.
of blastocysts(ratio)
- - 435 338(77%) 270(62%) 39±2.18 27-66
5 - 293 219(75%) 187(64%) 40±2.13 23-68
10 - 376 327(87%)* 300(80%)** 50±1.84** 25-83
- 10 408 261(64%) 204(58%) 35±2.55 23-52
10 10 312 221(71%) 184(59%) 41±3.36 29-67
Tab.1  牛磺酸对昆明小鼠胚胎在mKSOM培养液中体外发育的影响
Fig.2  不同浓度牛磺酸mKSOM培养液中的囊胚总细胞的荧光染色
Fig.3  牛磺酸对小鼠体外培养胚胎发育的影响
Fig.4  孕D4子宫角注射不同浓度牛磺酸转运抑制剂β-丙氨酸对着床的影响
孕D9胚胎数No. of embryos on D9(Mean±SE)
生理盐水
Normal saline		 β-丙氨酸(20 μg·mL-1)
β-ala		 β-丙氨酸(200 μg·mL-1)
β-ala
11.67±0.67 左侧Left 12.66±0.89 11.00±0.58
1.67±0.63** 右侧Right 10.67±0.43 4.67±0.55**
Tab.2  宫角注射β-丙氨酸对孕D9小鼠胚胎着床数的影响
[1] Guérin P, Guillaud J, Menezo Y.Hypotaurine in spermatozoa and genital secretions and its production by oviduct epithelial cells in vitro[J].Hum Reprod, 1995, 10(4):866-872.
doi: 10.1006/gyno.1995.1111 pmid: 7650134
[2] Harris S E, Gopichandran N, Picton H M, et al.Nutrient concentrations in murine follicular fluid and the female reproductive tract[J].Theriogenology, 2005, 64(4):992-1006.
doi: 10.1016/j.theriogenology.2005.01.004 pmid: 16054501
[3] Orensanz L M, Fernández I, Martín del Río R, et al.Gamma-aminobutyric acid in the rat oviduct[J].Adv Biochem Psychopharmacol, 1986, 42:265-274.
[4] Phoenix J, Wray S.Changes in human and rat uterine phosphoethanolamine and taurine with pregnancy and parturition[J].Exp Physiol, 1994, 79(4):601-604.
doi: 10.1113/expphysiol.1994.sp003794 pmid: 7946290
[5] Sturman J A.Taurine in development[J].Physiol Rev, 1993, 73(1):119-147.
[6] 强苏静, 陶凌云, 金益, 等.小鼠母体糖尿病(DM)对胚胎早期发育的影响[J].上海交通大学学报(农业科学版), 2012, 30(3):32-35.
doi: 10.3969/J.ISSN.1671-9964.2012.03.006
[7] Dumoulin J C M, Evers J L H, Bakker J A, et al.Temporal effects of taurine on mouse preimplantation development in vitro[J].Hum Reprod, 1992, 7(1):403-407.
doi: 10.1111/j.1365-2559.1992.tb00983.x pmid: 1587949
[8] Spindle A.Beneficialeffects of taurine on mouse zygotes developing in protein-free culture medium[J].Theriogenology, 1995, 44(6):761-772.
doi: 10.1016/0093-691X(95)00275-D pmid: 16727773
[9] Dumoulin J C M, Evers J L H, Bras M, et al.Positive effect of taurine on preimplantation development of mouse embryos in vitro[J].J Reprod Fertil, 1992, 94(2):373-380.
doi: 10.1530/jrf.0.0940373 pmid: 1593540
[10] Li R, Whitworth K, Lai L, et al.Concentration and composition of free amino acids and osmolalities of porcine oviductal and uterine fluid and their effects on development of porcine IVF embryos[J].Mol Reprod Dev, 2007, 74(9):1228-35.
doi: 10.1002/mrd.20682 pmid: 17342727
[11] Manjunatha B, Devaraj M, Gupta P S, et al.Effect of taurine and melatonin in the culture medium on buffalo in vitro embryo development[J].Reprod Domest Anim, 2009, 44(1):12-16.
doi: 10.1111/j.1439-0531.2007.00982.x pmid: 18507802
[12] 袁水桥, 罗光彬, 李东全, 等.牛磺酸在牛胚胎体外发育中的作用研究[J].沈阳农业大学学报, 2007, 38(4):552-555.
doi: 10.3969/j.issn.1000-1700.2007.04.023
[13] 郑云胜, 张家新, 陈玉琦, 等.氨基酸和牛磺酸对绵羊体外受精胚胎体外培养的影响[J].中国畜牧杂志, 2004, 40(3):16-18.
doi: 10.3969/j.issn.0258-7033.2004.03.007
[14] Devreker F, Van den Bergh M, Biramane J, et al.Effects of taurine on human embryo development in vitro[J].Hum Reprod, 1999, 14(9):2350-2356.
doi: 10.1093/humrep/14.9.2350 pmid: 10469709
[15] Guérin P E I, Mouatassim S, Ménézo Y.Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings[J].Hum Reprod Update, 2001, 7(2):175-189.
pmid: 3281591952098529535152922232222112846618
[16] Suzuki C, Yoshioka K, Sakatani M, et al.Glutamine and hypotaurine improves intracellular oxidative status and in vitro development of porcine preimplantation embryos[J].Zygote, 2007, 15(4):317-324.
doi: 10.1017/S0967199407004273 pmid: 17967211
[17] Choi Y H, Chung Y G, Walker S C, et al.In vitro development of equine nuclear transfer embryos:effects of oocyte maturation media and amino acid composition during embryo culture[J].Zygote, 2003, 11(1):77-86.
doi: 10.1017/S0967199403001102 pmid: 12625532
[18] 廖海艳, 庄远红.一氧化氮对小鼠胚胎附植期MMP-9 表达的影响[J].湖南农业科学, 2013(09):6-10.
doi: 10.3969/j.issn.1006-060X.2013.09.002
[19] 蔡理全, 曹宇静, 段恩奎.整合素αVβ3在小鼠胚胎植入中的作用[J].科学通报, 2000, 45(15):1639-1644.
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