为了解灰树花原基形成及子实体分化机制, 采用Illumina测序技术对灰树花菌丝体和原基进行了全转录组测序和数据分析, 对菌丝体和原基中的差异表达基因进行了研究。结果表明, 在菌丝体和原基中分别得到35788532个和32755254个高质量测序标签。差异表达基因分析表明, 两个文库中共有显著性差异表达的基因4094个, 其中在原基中上调、下调的基因数分别为1886和2208个, 只在原基中表达的基因284个。经Blastnr 比对, 在菌丝体与原基阶段差异表达的基因主要与酸性蛋白酶类、凝集素、细胞色素、NADPH-P450还原酶、酯酶、胺氧化酶、克拉维胺合成酶、糖苷水解酶家族相关。在原基中特异表达的基因主要与糖代谢、脂类代谢、核酸代谢及细胞膜、叶绿体膜有关。GO功能富集分析结果表明, 线粒体膜相关基因、谷氨酰胺代谢、脂肪酸生物合成相关基因均上调表达; Pathway 功能富集分析结果表明, 合成核糖体蛋白的基因均上调表达, 表明原基形成时细胞代谢增强, 蛋白质合成量增加。

The expression profiling of mycelium and primordium of Grifola frondosa was analyzed with high-throughput sequencing technology based on the Illumina Genome Analyzer platform.Totally 35788532 and 32755254 clean reads from mycelium and primordium libraries were obtained.Analysis of differentially expressed genes shows that there are 4094 genes differentially expressed between two libraries, and in the primordium library, 1886 of them are up-regulated while 2208 of them are down-regulated.There are 284 genes that are only expressed in the primordium library.With Blastnr comparison, genes differently expressed between two libraries are found to be related to acid protease and lectin, cytochrome, NADPH-P450 reductase, esterase, amine oxidase, clavaminate synthase, glycoside hydrolase.Genes only expressed in the primordium are found to be related to glucose, lipid, nucleic acids metabolism and cell and chloroplast membrane structure.Gene ontology functional enrichment analysis reveals that most genes related to mitochondrial membrane, glutamine family amino acid metabolic process, fatty acid biosynthetic process are up-regulated.Pathway enrichment analysis shows that genes related to ribosome are all up-regulated, and this indicates that when primordium is developed the cell metabolism speeds up and the protein synthesis increases.