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2016 , Vol. 34 >Issue 1: 11 - 15

DOI: https://doi.org/10.3969/J.ISSN.1671-9964.2016.01.003

Orginal Article

Effectof Taurine on Development and Implantation of Kunming Mouse Early Embryos

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  • 1. Biological and Agricultural Engineering College ofWeifang University, Weifang 261061, China
    2. Key Laboratory of Biochemistry & Molecular Biology in Universities of Shandong, Weifang University, Weifang 261061 China
    3. College of Chemistry and Chemical Engineering, Weifang University, Weifang 261061, China

Received date: 2015-02-01

  Online published: 2016-03-08

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Abstract

The effects of taurine on development and implantation of Kunming mouse early embryos were investigated.The embryos were cultivated in modified potassium simplex optimized medium(mKSOM)with different concentration of taurine.The percentage of 4-cell, blastocysts and average total cell number of blastocysts were calculated after 24 h and 72 h.Cornua uteri injection technique was used to investigate the effects of β-alaline(β-ala, taurine transport inhibitor)on embryo implantation.The results showed that there were significant differences between 10 mmol/L taurine treatment and control in the percentage of 4-cell(P<0.05), blastocysts and total cell number of blastocysts(P<0.01).Meanwhile, injection of β-ala(20 μg/mL)into uterine horn significantly reduced the number of implanted embryos on D9.This study explicated the effects of taurine on the development and implantation of early Kunming mouse embryos, providing a useful reference for further uncovering of mechanism of embryo development and implantation.

Key words： taurine; embryo development; implantation

Cite this article

Jing-jing FAN, Qiu-kai ZHENG . Effectof Taurine on Development and Implantation of Kunming Mouse Early Embryos[J]. Journal of Shanghai Jiaotong University (Agricultural Sciences), 2016 , 34(1) : 11 -15 . DOI: 10.3969/J.ISSN.1671-9964.2016.01.003

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