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Effectof Taurine on Development and Implantation of Kunming Mouse Early Embryos
Received date: 2015-02-01
Online published: 2016-03-08
The effects of taurine on development and implantation of Kunming mouse early embryos were investigated.The embryos were cultivated in modified potassium simplex optimized medium(mKSOM)with different concentration of taurine.The percentage of 4-cell, blastocysts and average total cell number of blastocysts were calculated after 24 h and 72 h.Cornua uteri injection technique was used to investigate the effects of β-alaline(β-ala, taurine transport inhibitor)on embryo implantation.The results showed that there were significant differences between 10 mmol/L taurine treatment and control in the percentage of 4-cell(P<0.05), blastocysts and total cell number of blastocysts(P<0.01).Meanwhile, injection of β-ala(20 μg/mL)into uterine horn significantly reduced the number of implanted embryos on D9.This study explicated the effects of taurine on the development and implantation of early Kunming mouse embryos, providing a useful reference for further uncovering of mechanism of embryo development and implantation.
Key words: taurine; embryo development; implantation
Jing-jing FAN, Qiu-kai ZHENG . Effectof Taurine on Development and Implantation of Kunming Mouse Early Embryos[J]. Journal of Shanghai Jiaotong University (Agricultural Sciences), 2016 , 34(1) : 11 -15 . DOI: 10.3969/J.ISSN.1671-9964.2016.01.003
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