Home  中文

Journal of Shanghai Jiaotong University (Agricultural Sciences) >

2016 , Vol. 34 >Issue 1: 36 - 44

DOI: https://doi.org/10.3969/J.ISSN.1671-9964.2016.01.007

Orginal Article

Effect of Bacterial Community Structure Diversity with Different Carbon and Nitrogen Organic Materials Treatments in Organic Vegetable Soil

Expand
  • 1.Key Laboratory of Urban Agriculture(South), Ministry of Agriculture, School of Agriculture and Biology, Shanghai Jiaotong University; Shanghai 200240, China
    2.School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney 2052, Australia

Received date: 2014-12-22

  Online published: 2016-03-08

Fold

Abstract

Through the simulation test, PCR-DGGE method was used to investigate the effects of different carbon and nitrogen organic materials on bacterial community structure of organic vegetable production system soil.Results showed that bacterial community structure of conventional and organic production system soils were significantly different.By analyzing the Shannon-Wiener diversity index, overall the bacterial diversity of organic background soil treatments was higher than conventional background soil treatments.The bacterial diversity of organic production system soil with straw(OS)was the highest.Bacterial diversity decrease or increase after adding urea or alfalfa respectively.Conventional and organic production system soils were divided into two ethnic clusters by clustering analysis.Phylogenetic tree showed that the 30 closely related species were attributed to Proteobacteria, Acidobacteria, Actinobacteria, Firmicutes, Verrucomicrobia.The band B13 of CA(conventional production system soil with alfalfa)and the common band B28 of organic background treatment had the highest homology with Bacillus and Pseudomonas respectively.

Key words： straw; alfalfa; urea; organic soil; PCR-DGGE; bacterial community structure

Cite this article

Hao YANG, Gonzalez Perez Pablo, Rui ZHANG, Jun YE, Bao-song CAI, Dan-feng HUANG . Effect of Bacterial Community Structure Diversity with Different Carbon and Nitrogen Organic Materials Treatments in Organic Vegetable Soil[J]. Journal of Shanghai Jiaotong University (Agricultural Sciences), 2016 , 34(1) : 36 -44 . DOI: 10.3969/J.ISSN.1671-9964.2016.01.007

References

[1] Sun H, Deng S, Raun W.Bacterial community structure and diversity in a century-old manure-treated agroecosystem[J].Applied and Environmental Microbiology, 2004, 70(10):5868-5874.
[2] Wei D, Yang Q, Zhang J Z, et al.Bacterial community structure and diversity in a black soil as affected by long-term fertilization[J].Pedosphere, 2008, 18(5):582-592.
[3] 毕明丽, 宇万太, 姜子绍, 等.施肥和土壤管理对土壤微生物生物量碳、氮和群落结构的影响[J].生态学报, 2010, 30(1):32-42.
[4] Chu H, Lin X, Fujii T, et al.Soil microbial biomass, dehydrogenase activity, bacterial community structure in response to long-term fertilizer management[J].Soil Biology and Biochemistry, 2007, 39(11):2971-2976.
[5] 许仁良, 王建峰, 张国良, 等.秸秆、有机肥及氮肥配合使用对水稻土微生物和有机质含量的影响[J].生态学报, 2010, 30(13):3584-3590.
[6] 邢德峰, 任南琪, 宋佳秀, 等.不同16SrDNA靶序列对DGGE分析活性污泥群落的影响[J].环境科学, 2006, 27(7):1424-1428.
[7] Li Z, He L, Miao X.Cultivable bacterial community from South China Sea sponge as revealed by DGGE fingerprinting and 16S rDNA phylogenetic analysis[J].Current microbiology, 2007, 55(6):465-472.
[8] 陈哲, 陈春兰, 秦红灵, 等.化肥对稻田土壤细菌多样性及硝化、反硝化功能菌组成的影响[J].生态学报, 2009, 29(11):6142-6147.
[9] 徐永刚, 宇万太, 马强, 等.长期不同施肥制度对潮棕壤微生物生物量碳、氮及细菌群落结构的影响[J].应用生态学报, 2010, 21(8):2078-2085.
[10] Sarathchandra S, Ghani A, Yeates G, et al.Effect of nitrogen and phosphate fertilisers on microbial and nematode diversity in pasture soils[J].Soil Biology and Biochemistry, 2001, 33(7):953-964.
[11] Yao S, Merwin I A, Bird G W, et al.Orchard floor management practices that maintain vegetative or biomass groundcover stimulate soil microbial activity and alter soil microbial community composition[J].Plant and soil, 2005, 271(1-2):377-389.
[12] 刘丽, 徐明恺, 汪思龙, 等.杉木人工林土壤质量演变过程中土壤微生物群落结构变化[J].生态学报, 2013, 33(15):4692-4706.
[13] 王曙光, 侯彦林.尿素肥斑扩散对土壤微生物群落结构的影响[J].生态学报, 2004, 24(10):2269-2274.
[14] Abbasi M W, Ahmed N, Zaki M J, et al.Potential of Bacillus species against Meloidogyne javanica parasitizing eggplant(Solanum melongena L.)and induced biochemical changes[J].Plant and Soil, 2014, 375(1-2):159-173.
[15] 任小平, 谢关林, 王笑.铜绿假单胞菌ZJ1999对水稻纹枯病的防治及其在水稻上的定殖[J].中国生物防治, 2006, 22(01):54-57.
[16] 楼兵干, 张炳欣, Ryder M.铜绿假单胞菌株CR56在黄瓜和番茄根围的定殖能力[J].浙江大学学报(农业与生命科学版), 2001, 27(2):67-70.
[17] Kloepper J W, Leong J, Teintze M, et al.Enhanced plant growth by siderophores produced by plant growth-promoting rhizobacteria[J].Nature, 1980, 286(5776):885-886.
[18] Topp E, Mulbry W M, Zhu H, et al.Characterization of s-triazine herbicide metabolism by a Nocardioides sp.isolated from agricultural soils[J].Applied and Environmental Microbiology, 2000, 66(8):3134-3141.
[19] 姜钰, 董怀玉, 徐秀德, 等.放线菌在植病生防中的研究进展[J].杂粮作物, 2005, 25(5):329-331.
[20] Muyzer G, De Waal E C, Uitterlinden A G.Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA[J].Applied and Environmental Microbiology, 1993, 59(3):695-700.
Options
Outlines

/

〈 〉

Copyright © 2015 Journal of Shanghai Jiaotong University (Agricultural Sciences), All Rights Reserved.
Powered by Beijing Magtech Co. Ltd