组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2010, Vol. 6 ›› Issue (1): 9-13.doi: 10.3969/j.issn.1673-0364.2010.01.003

• 论著 • 上一篇    下一篇

SVF-PLGA联合脂肪移植体内构建组织工程化软组织充填材料的初步研究

麦凯欣，朱辉，崔永言   

  1. 北京大学深圳医院整形美容外科; 汕头大学医学院
  • 发布日期:2020-07-23

Preliminary Study of Co-Transplantation of SVF-PLGA with Fat Complex on the Construction of Tissue Engineering Implant

MAI Kaixin, ZHU Hui, CUI Yongyan   

  1. Department of Plastic Surgery; Peking University Shenzhen Hospital; Shenzhen 518036; China.
  • Published:2020-07-23

摘要： 目的采用组织工程方法,探讨生物可降解材料、自体脂肪作为细胞外基质,脂肪组织基质血管成分(SVF)作为种子细胞构建复合移植物,即刻回植,体内培养并用于软组织充填的可行性及安全性。方法取雄性新西兰大白兔40只,随机分为4组:实验组A、B(各10只)以两种混合方式构建SVF-脂肪-PLGA复合移植物,实验组A将SVF先与脂肪混合再种植于支架上,实验组B将SVF与支架充分混合,再用脂肪组织包埋;对照组A(n=10)的移植物为预处理后的空白支架;对照组B(n=10)的移植物为SVF-PLGA复合物。各组均将植人物移植至实验兔背部皮下与肌肉浅筋膜之间,于术后2个月、4个月取材,进行大体观察、组织切片、HE染色和油红染色,了解支架降解情况和组织工程化软组织充填材料构建情况,观察期间记录术后所出现的各种并发症和不良反应。结果术后4个月时,各组均未发生切口感染、硬结形成、移植物移位和排斥等并发症。实验组B中有1例切口裂开、2例脂肪液化。大体观察和HE染色、油红染色可见形成的组织工程化软组织填充材料主要成分为血管化脂肪组织,未发现肿瘤样细胞,外周形成纤维膜。4个月后PLGA支架完全降解,实验组B的脂肪存活率较实验组A和对照组B高。结论构建SVF-脂肪-PLGA复合物软组织充填材料是可行的,具有安全、可操作性强、耗时少、脂肪存活率高、塑形性好等优点。

关键词: 移植, 软组织, 充填材料, 组织工程, 支架, 脂肪

Abstract: Objective To evaluate the feasibility and safety of co-transplantation of SVF-PLGA with fat complex on the construction of tissue engineered implant.Methods Forty adult New Zealand male rabbits were randomly divided into four groups.In the experimental group A(n=10),implant was constructed by use of SVF-PLGA with fat complex and in the group B (n=10).SVF-PLGA complex buried by adipose tissue was used.In the control group C(n=10) only PLGA and group D(n=10), SVF-PLGA complex was used.All the complex were translated between skin and superficial fascia.Tissue samples obtained were analyzed with gross observation,HE stain and red oil stain at 4 months postoperatively.Results The tissue engineered implant was mainly vascularized adipose tissue without tumor cell.PLGA scaffolds were degraded completely 4 months later. The survival rate of fat in experimental group B was higher than in the group A and in the control group B.Conclusion SVF-PLGA with fat complex on soft tissue with the advantage of high survival rate of fat.

Key words: Transplantation, Soft tissue, Implant, Tissue engineer, Scaffold, Fat

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