组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2020, Vol. 16 ›› Issue (3): 237-240.doi: 10.3969/j.issn.1673-0364.2020.03.016

• 论著 • 上一篇    下一篇

自体骨髓单核细胞移植对兔退行性病变后椎间盘的修复作用

崔基浩1, 李创1(), 马文斌2, 李春1, 徐万宏1   

  1. 1. 528315 广东省佛山市 广州医科大学附属顺德医院(佛山市顺德区乐从医院)骨科
    2. 528315 广东省佛山市 广州医科大学附属顺德医院,超声诊断科
  • 收稿日期:2020-04-01 修回日期:2020-04-28 出版日期:2020-07-27 发布日期:2020-06-26
  • 基金资助:
    佛山市自筹经费类科技计划项目(1920001000936)

Regeneration of Degenerated Intervertebral Disc with Autologous Bone Marrow Mononuclear Cells in Rabbit Models

Jihao CUI1, Chuang LI1(), Wenbin MA2, Chun LI1, Wanhong XU1   

  1. 1. Department of Orthopedic Surgery, Affiliated Shunde Hospital of Guangzhou Medical University, Foshan 528315, China
    2. Department of Ultrasound Diagnosis, Affiliated Shunde Hospital of Guangzhou Medical University, Foshan 528315, China
  • Received:2020-04-01 Revised:2020-04-28 Online:2020-07-27 Published:2020-06-26

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摘要：

目的 研究自体骨髓单核细胞移植对兔退行性病变后椎间盘的修复作用。

方法 选取新西兰大白兔12只,随机分为对照组(n=6)与治疗组(n=6)。取新西兰大白兔髂后上棘骨髓,密度梯度离心法提取骨髓单核细胞。两组实验兔麻醉后使用16 G穿刺针进行L4/5、L5/6、L6/7椎间盘穿刺造模。治疗组将骨髓单核细胞注入到穿刺造模的椎间盘中,对照组不予治疗。第4、8、24周分别进行椎间盘的影像学(X线和MR)与组织学观察。

结果 在椎间隙高度维持方面,随着周数的增加,治疗组优于对照组;在MR T2W1加权像的信号强度及组织形态方面,治疗组明显优于对照组。组织学观察显示,与治疗组相比,对照组中的髓核细胞数量减少,胞外基质收缩、不均。

结论 自体骨髓单核细胞移植对兔退行性病变后的椎间盘具有一定的修复再生作用。

关键词: 椎间盘, 自体骨髓单核细胞, 退行性病变, 再生

Abstract:

Objective To investigate the regenerative effect of autologous bone marrow mononuclear cells on degenerated intervertebral disc in rabbit models.

Methods A total of 12 New Zealand white rabbits were selected. The rabbits were randomly divided into two groups: the control group (n=6) and the treatment group (n=6). Bone marrow was extracted from the posterior superior iliac spine of the New Zealand white rabbit, and autologous bone marrow mononuclear cells were extracted by density gradient centrifugation. L4/5, L5/6 and L6/7 intervertebral disc puncture models were made with 16 G needle after anesthesia in the two groups of rabbits. Autologous bone marrow mononuclear cells were injected into the punctured disc of the treatment group and no treatment was done in the control group. Then the rabbits in the two groups were raised under the same conditions. Imaging (X-ray and MR) and histological observation of the disc were performed at weeks 4, 8, and 24, respectively.

Results In terms of maintaining the height of intervertebral space, the treatment group was superior to the control group as the number of weeks increased. In terms of signal strength of MR T2W1 and histological morphology, the treatment group was significantly better than that of the control group. Histological observation showed that compared with the treatment group, the number of nucleus pulposus cells in the control group was reduced, and the extracellular matrix was contracted and uneven.

Conclusion Bone marrow monoculear cells transplantation can regenerate the degenerated intervertebral disc in rabbit models.

Key words: Intervertebral disc, Autologous bone marrow mononuclear cell, Degeneration, Regeneration

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