组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2022, Vol. 18 ›› Issue (4): 305-.

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泛醌对大鼠背部皮瓣缺血再灌注损伤的作用与影响

  

  • 出版日期:2022-08-01 发布日期:2022-09-06

Effect of ubiquinone on ischemia-reperfusion injury of rat dorsal skin flap

  • Online:2022-08-01 Published:2022-09-06

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摘要： 目的　通过大鼠背部皮瓣模型探究泛醌在缺血再灌注损伤过程中的作用。方法　选用120只健康成年雄性SD大鼠，随机分为假手术组、实验组和对照组，每组40只。在大鼠背部设计一个9.0 cm×6.0 cm的皮瓣，并结扎6条供血血管中的5条，实验组及对照组进行缺血再灌注损伤造模。术后实验组给予泛醌50 mg/kg；假手术组及对照组每天给予等量的食用油，灌胃体积均为每天5 mL/kg。于模型建立后的第1、3、5、7天各组分别随机处死10只大鼠，观察各组皮瓣存活率、皮瓣组织HE染色、皮瓣组织SOD活性及MDA含量。结果　统计分析各组皮瓣存活率、皮瓣组织HE染色、皮瓣组织SOD活性及MDA含量，除第7天假手术组和实验组皮瓣组织MDA含量无统计学差异外（P＞0.05），其余各时间点，假手术组优于实验组、实验组优于对照组（P＜0.05）。结论　泛醌可有效减轻大鼠背部超大皮瓣的缺血再灌注损伤情况。

关键词: 皮瓣移植, 　缺血再灌注损伤, 　泛醌, 　抗氧化剂, 　超氧化物歧化酶, 　丙二醛

Abstract: Objective　To explore the role of ubiquinone in ischemic reperfusion injury  by rat dorsal flap model. Methods　A total of 120 healthy adult male SD rats were randomly divided into three groups: sham surgery group, experimental group and control group, with 40 rats in each group. A flap of 9.0 cm×6.0 cm was designed on the back of the rat and five of the six blood supply vessels were ligated, and the experimental group and the control group were molded. The experimental group received 50 mg/kg ubiquinone; The sham surgery group and the control group were given the same amount of cooking oil every day, and the gastric volume was 5 mL/kg per day. On the 1st, 3rd, 5th and 7th days after the model was established, 10 rats were randomly sacrificed in each group to observe the corresponding indicators: the flap survival rate, flap tissue HE staining, flap tissue SOD activity and MDA content. Results　The flap survival rate, flap tissue HE staining, flap tissue SOD activity and MDA content of flap tissue were statistically analyzed. The sham operation group was superior to the experimental group and the experimental group was superior to the control group at all time points (P<0.05) except for the MDA content of skin flap between the sham operation group and the experimental group on the 7th day (P>0.05). Conclusion　Ubiquinone can effectively alleviate ischemia-reperfusion injury of oversized flaps on the back of rats.

Key words: Flap transplantation, 　Ischemic reperfusion injury, 　Ubiquinone, 　Antioxidant, 　Superoxide dismutase, 　Malondialdehyde