组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2024, Vol. 20 ›› Issue (4): 416-.

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静电纺丝制备的聚己内酯/还原氧化石墨烯神经导管基底膜的生物相容性研究

  

  • 出版日期:2024-08-01 发布日期:2024-09-11

Biocompatibility of PCL/RGO nerve duct basement membrane prepared by electrospinning

  • Online:2024-08-01 Published:2024-09-11

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摘要：

目的　探索 5 种不同含量的聚己内酯/还原氧化石墨烯（Polycaprolactone/reduced graphene oxide，PCL/RGO） 神经导管基底膜材料与 PC12 细胞的体外生物相容性。方法　应用静电纺丝技术制备 5 种不同 RGO 含量的 PCL/ RGO 神经导管基底膜样材料，并与 PC12 细胞共培养。使用 CCK-8 法测定 PC12 细胞的增殖情况，扫描电镜观察细胞 的黏附与生长情况，活死细胞染色观察细胞活性。结果　5 组材料所对应的细胞增殖率有差异，但毒性分级均为 0级（无细胞毒性），其中 0.75%RGO/PCL 组的细胞增殖情况最佳。5 组材料培养 7 d 后，电镜观察显示细胞黏附生长明 显较 1 d、3 d 增多，并铺满了材料表面。活死细胞染色结果显示，各组细胞存活率均在 90% 以上。结论　5 种不同RGO 含量的 PCL/RGO 复合材料均具有良好的生物相容性，尤以 0.75%RGO/PCL 在促进细胞增殖、黏附方面有比较大 的优势。

关键词: 静电纺丝, 　聚己内酯, 　还原氧化石墨烯, 　PC12细胞, 　生物相容性

Abstract: Objective　 To explore the in vitro biocompatibility of five different contents of polycaprolactone/reduced graphene oxide （PCL/RGO） nerve duct basement membrane materials with PC12 cells. Methods　Five kinds of PCL/RGO nerve duct basement membrane materials with different RGO contents were prepared by electrospinning technique and cocultured with PC12 cells. The proliferation of PC12 cells was determined by CCK-8 method， the adhesion and reproduction of PC12 cells were observed by scanning electron microscopy， and the cell activity was observed by Live/Dead fluorescence staining. Results　The cell proliferation rate of the five groups was different， but the toxicity grade was 0（ no cytotoxicity）， and the cell proliferation of 0.75%RGO/PCL group was the best. After 5 groups of materials were cultured for 7 days， the cell adhesion growth was significantly increased under electron microscope compared with 1 day and 3 days， and the surface of the materials was fully coated. The Live/Dead fluorescence staining results showed that the survival rate of cells in all groups was above 90%. Conclusion　Five kinds of PCL/RGO composites with different RGO contents all have good biocompatibility， and the composite materials with 0.75%RGO/PCL ratio have a relatively large advantage in promoting cell proliferation and adhesion.

Key words: 　Electrospinning, 　Polycaprolactone, 　Reduced graphene oxide, 　PC12 cells, 　Biocompatibility