组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2025, Vol. 21 ›› Issue (1): 26-.

• • 上一篇    下一篇

维替泊芬聚乳酸纳米凝胶对大鼠全层皮肤损伤创面的作用研究

  

  • 出版日期:2025-02-25 发布日期:2025-03-06

Study on the effect of veteporfin polylactic acid nanogel on full-thickness skin injury wound in rats

  • Online:2025-02-25 Published:2025-03-06

PDF (PC)

3

摘要：

目的　将维替泊芬聚乳酸纳米凝胶（HA/VP-PLA）应用于大鼠皮肤损伤创面，评估HA/VP-PLA在促进创面愈合及减轻瘢痕方面的作用与优势。方法 50只雄性SD大鼠背部建立皮肤全层缺损创面，创面皮缘用无菌钢环缝合固定。将大鼠随机分组为透明质酸凝胶组、空白对照组、HA/VP-PLA纳米凝胶组、外用重组人粒细胞巨噬细胞刺激因子凝胶（金扶宁）组、湿润烧伤膏（美宝）组，观察各组大鼠创面愈合情况。分别在术后第9天和第18天取材，并进行HE染色、Masson染色、天狼猩红染色；免疫组化检测α-SMA、CD31的表达情况。结果 HA/VP-PLA纳米凝胶组、金扶宁组、美宝组的大鼠创面愈合率均明显高于空白对照组，且具有显著性差异。HE染色显示，与空白对照组相比，HA/VP-PLA纳米凝胶组、金扶宁组、美宝组创面炎症浸润少，修复更快。Masson染色显示HA/VP-PLA纳米凝胶组比空白对照组的胶原纤维排列疏松，密度小，比金扶宁、美宝组胶原排序也更好。第18天HA/VP-PLA纳米凝胶组纤维化程度明显低于空白对照组、透明质酸组，且具有显著性差异。天狼猩红染色显示，第 18天Ⅰ/Ⅲ胶原比例 HA/VP-PLA 纳米凝胶组最低，与空白对照组、美宝组相比具有显著性差异。第9天时HA/VP-PLA纳米凝胶组的CD31表达最高，与其他各组相比具有显著性差异。第9天和第18天时HA/VP-PLA纳米凝胶组的α-SMA表达量最低，与空白对照组相比具有显著性差异，第18天时与金扶宁组相比也有显著性差异。结论 HA/VP-PLA纳米凝胶在促进大鼠创面上皮化效果方面与重组人粒细胞巨噬细胞刺激因子凝胶和湿润烧伤膏相近，在降低组织纤维化、减少瘢痕形成方面优于后两者，具有临床转化和应用前景。

关键词:

Abstract:

Objective To explore the application of verteporfin polylactic acid （HA/VP-PLA） nanogel on full-thickness skin wounds in rats and compare its performance with existing products in terms of healing efficacy and scar reduction. Methods Fifty male rats were selected， and full-thickness skin defects were created on their backs. The wound margins were sutured and secured with sterile steel rings. According to different wound medication regimens， the rats were randomly assigned to five groups： hyaluronic acid gel group， blank control group， HA/VP-PLA nanogel group， Jinfuning group， and MEBO group. Wound healing was monitored， and tissue samples were collected on days 9 and 18 post-surgery for hematoxylin-eosin， Masson， and picrosirius red staining. Immunohistochemical analyses were conducted to measure the expression of α-SMA and CD31. Results The healing rates in the HA/VP-PLA nanogel， Jinfuning， and MEBO groups were significantly greater than those in the blank control group. HE staining revealed less inflammatory infiltration and quicker repair in these groups compared to the control. Masson staining indicated that the HA/VP-PLA nanogel group had  looser and less dense collagen fibers than those in the control group， with better-organized collagen compared to the Jinfuning and MEBO groups. The extent of fibrosis on the 18th day was markedly reduced in the HA/VP-PLA group compared to the blank and hyaluronic acid gel groups. Sirius red staining showed that the ratio of type Ⅰ to Ⅲ collagen was lowest in the HA/ VP-PLA group， showing significant differences with both the control and MEBO groups. On day 9， CD31 expression was highest in the HA/VP-PLA group， significantly differing from other groups. Similarly，α-SMA expression was lowest on days 9 and 18 in the HA/VP-PLA group， with significant differences noted against the blank control group and the Jinfuning group on day 18. Conclusion The HA/VP-PLA nanogel is effective in promoting wound epithelialization， comparable to the Jinfuning and MEBO groups， but superior in reducing tissue fibrosis and scar formation， which had clinical transformation and application prospects.

Key words: