组织工程与重建外科杂志 ›› 2025, Vol. 21 ›› Issue (4): 353-.

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四氧化三锰纳米酶促进糖尿病创面愈合的机制研究

  

  • 出版日期:2025-08-01 发布日期:2025-09-08

Study of the mechanism of trimanganese tetroxide nanoenzyme for diabetic wound healing

  • Online:2025-08-01 Published:2025-09-08

摘要:

 目的 开发一款通过清除活性氧(ROS)以促进糖尿病创面愈合的伤口敷料,并探究其作用机制。方法 制备四氧化三锰纳米酶,并使用材料学检测方法对其结构与性能进行表征;通过体外细胞实验检测纳米酶的生物相容性和抗氧化应激的能力;应用糖尿病小鼠皮肤缺损模型,通过大体观、组织学等对材料促进创面愈合的性能进行验证。结果 四氧化三锰纳米酶尺寸约为5 nm,表面同时存在Mn2+和Mn3+,结晶度、分散性与酶活性良好,浓度低于8 μg/mL时
无细胞毒性。体外实验中,加入ROS后M1型标志物增高5.1~9.7倍,加入四氧化三锰纳米酶后,M1型标志物下调明显
,与对照组无统计学差异(P>0.05)。体内实验第14天时,材料组创面仅剩0.3%未愈合,对照组8.5%,空白对照组3.7%,具有显著统计学差异(P<0.000 1)。结论 本研究成功制备四氧化三锰纳米酶,其具有良好的安全性与有效性,体内、外实验结果表明,材料能有效清除ROS,通过缓解创面中多种不利因素的影响,最终促进创面愈合,为创面敷料提供了一种新的选择。

关键词:

Abstract:

Objective To develop a wound dressing that promotes diabetic wound healing by removing ROS and explore its mechanism. Methods Manganese tetraoxide nanozyme was prepared, and its structure and properties were characterized by materials science testing. The biocompatibility and anti-oxidative stress ability of nanozyme were tested by in vitro cell  experiments. The skin defect model of diabetic mice was used to verify the performance of the material in promoting wound healing by gross observation and histology. Results Manganese tetraoxide nanozyme is about 5 nanometers in size, with both Mn2+and Mn3+on the surface. It has good crystallinity, dispersibility and enzyme activity, and has no cytotoxicity at concentrations below 8 μg/mL. In in vitro experiments, the M1 marker increased significantly by 5.1-9.7 times after the addition of ROS. After the addition of manganese tetraoxide nanozyme, the M1 marker was significantly downregulated, with no difference from the control group (P>0.05). On the 14th day of the in vivo experiment, only 0.3% of the wound surface remained in the material group,8.5% in the control group, and 3.7% in the blank control group, with significant statistical differences (P<0.000 1). Conclusion Manganese tetraoxide nanozymes were successfully prepared in this study, which
have good safety and efficacy. The combined in vivo and in vitro experimental results show that the material can effectively remove ROS, alleviate the effects of various adverse factors in the wound, and ultimately promote wound healing, providing a new choice for wound dressings in clinical practice.

Key words:

healing