目的 探索激光打孔脱细胞基质诱导骨髓间充质干细胞(BMSCs)于动物体内构建组织工程气管软骨的可行性。方法 应用激光打孔联合脱细胞技术制备激光打孔脱细胞基质(LDTM)作为支架材料。抽取兔骨髓,分离培养BMSCs,接种于LDTM支架后植入兔皮下。体内培养12周后,行大体观察、组织学检测、生物力学及生物化学定量检测。结果 细胞均匀分布在激光微孔和支架表面,并且该复合物能够较好地维持原始的管状结构,形成了富有弹性的瓷白色软骨样组织。HE染色可见典型的软骨陷窝出现,说明成熟软骨组织形成。Safranin-O染色证实有蛋白聚糖基质产生。生物力学和生物化学检测显示新生气管组织均接近正常气管组织。结论 LDTM可以促进软骨再生并在动物体内诱导BMSCs构建组织工程气管软骨。

Objective To explore the feasibility of laser drilling decellularized trachea matrix(LDTM)inducing bone marrow mesenchymal stem cells(BMSCs)for constructing tissue-engineered tracheal cartilage in rabbits.Methods LDTM was fabricated by laser drilling and decellularization technique.BMSCs were harvested from bone marrow of rabbits and cultured in vitro.After the expanded cells were seeded onto the scaffolds,the cell-scaffold constructs were implanted into the subcutaneous of rabbits.Gross observation,histological observation and quantitative evaluation of neocartilage test were carried out after cultured for 12 weeks in vivo.Results The cells were evenly distributed into micropores and surface of the scaffold,and the construct maintained the original tubular structure with a flexible white cartilage-like tissue.HE staining showed typical mature lacuna,suggesting the formation of mature cartilage tissue.Safranin-O staining showed abundant proteoglycan.Biomechanical and biochemistry analyses indicated that neocartilage was close to or even better than native trachea cartilage.Conclusion LDTM promote cartilage regeneration and can induce BMSCs to construct tissue-engineered tracheal cartilage in rabbits.