目的探讨M2型巨噬细胞和Th2细胞因子在增生性瘢痕形成过程中发挥作用的具体机制。方法运用权重基因共表达网络分析(WGCNA)对小鼠增生性瘢痕全基因组表达数据GSE26390进行处理,获得与M2型巨噬细胞、Th2细胞因子以及纤维化高度相关的共表达基因模块和其中起到关键作用的靶基因。结果确定以下8个基因为M2型巨噬细胞、Th2型细胞因子参与增生性瘢痕形成的核心调控基因:CLEC4A、CYBA、LOXL2、Hcvn1、NCKAP1L、PRRX2、LGALS9、CMTM3。结论以上8个基因涉及巨噬细胞和T细胞参与免疫应答的调节、细胞外基质形成、组织修复等过程,是临床预防和治疗增生性瘢痕的潜在新靶点。

Objective To explore the specific mechanism of M2 macrophages and Th2 cytokines in the process of hypertrophic scar formation.Methods The weighted gene co-expression network analysis(WGCNA)was used to analysis the genome-wide expression data of mouse hypertrophic scar GSE26390,and the co-expression gene module and core regulatory genes highly correlated with M2 macrophage,Th2 cytokine and fibrosis were obtained.Results The following 8 genes were identified as core regulatory genes of M2 macrophages and Th2 type cytokines involved in the formation of hypertrophic scar:CLEC4A,CYBA,LOXL2,Hcvn1,NCKAP1L,PRRX2,LGALS9,CMTM3.Conclusion The above eight genes are involved in the regulation of immune response,extracellular matrix formation,tissue repair and other processes related to macrophage and T cells,and are potential new targets for prevention and treatment of hypertrophic scars.