目的 观察骨髓间充质干细胞预处理对于七氟烷导致的原代神经元细胞凋亡、细胞活性,以及TNFα水平的影响。方法 采用Transwell构建非接触性共培养体系,完成骨髓间充质干细胞对原代神经元的预处理。待原代神经元接受4%七氟烷暴露6 h后,观察其细胞形态;利用Western Blot检测凋亡程度;利用MTT和LDH检测细胞活性改变;利用ELISA检测TNFα水平。结果 BMSC预处理可以减少七氟烷对原代神经元导致的形态学改变,降低由七氟烷诱导的原代神经元凋亡(P<0.05)和细胞活性降低(P<0.05),以及TNFα大量释放(P<0.05)。结论 BMSC预处理可以缓解七氟烷暴露导致的神经毒性损伤。

Objective To explore the effects of bone marrow mesenchymal stem cells(BMSCs)on apoptosis,cell viability and the level of TNFα of primary cultured neuron. Methods BMSCs and primary cultured neuron were cultured in non-contact co-culture system by Transwell plate.After the exposure to 4% sevoflurane for 6 hours,the morphological changes of primary cultured neuron were observed. Western Blot was used to analyze cell apoptosis; MTT and LDH were used to detect cell viability;ELISA was used to determine the level of TNFα.Results The cell morphological changes, cell apoptosis, cell viability decline and TNFα release of primary cultured neuron induced by sevoflurane were all decreased after BMSCs preprocessing(P<0.05).Conclusion BMSCs preprocessing can suppress the neurotoxic injury induced by sevoflurane.