目的研究压力大小可控的培养系统,以实现组织工程化生物膜的动态培养。方法根据帕斯卡定律的基本原理,设计培养基压力的控制方法,建立由培养皿、蠕动泵、压力控制模块、主控器与检测模块等组成的组织工程化生物膜动态培养系统。结果该系统可实现培养基于0.12～19.76 mmHg压力范围内的控制。结论该系统为适宜压力范围内的生物膜培养提供所需的压力环境,为组织工程化生物膜动态培养技术提供新思路。

Objective To investigate the culture system that can control the culture pressure and realize the dynamic culture of tissue engineered biofilm. Methods According to the basic principle of Pascal's law, a control method of media pressure is designed, and a tissue engineered biofilm dynamic culture system is established. The system is composed of culture dish, peristaltic pump, pressure control module, main controller and detection module. Results This system can realize the pressure control of the culture with a pressure range of 0.12-19.76 mmHg. Conclusion This system provides the pressure environment of biofilm in suitable pressure range required for culture. It provides a new method for tissue engineered biofilm dynamic culture technology.