目的 观察丹参对3T3-L1细胞成脂分化和增殖的影响,为丹参应用于自体脂肪移植提供实验依据。方法将含有不同终浓度丹参注射液的完全培养液(0.6 g/L、0.3 g/L、0.15 g/L、0.075 g/L、0.04 g/L、0.02 g/L)分别加入诱导分化的3T3-L1细胞,待细胞分化至成熟脂肪细胞时,利用CCK-8法检测细胞增殖活力;利用油红O染色法、Image-pro plus6.0软件观察脂肪细胞内脂质的聚积;利用甘油三酯GPO-POD法测定脂肪细胞的脂质含量。结果 丹参可促进3T3-L1细胞增殖,且呈一定的时间、剂量依赖关系:加药时间点越早,对3T3-L1细胞增殖的促进作用越大;丹参浓度越大,对3T3-L1细胞增殖的促进作用越大。丹参同时也促进了3T3-L1细胞的成脂分化,增加细胞内脂质的聚积,并呈一定的剂量依赖关系。结论 丹参能够促进3T3-L1细胞的成脂分化以及增殖,使成熟脂肪细胞的数量增加。

Objective To explore the effect of Salvia miltiorrhiza on proliferation and differentiation of 3T3-L1 cells, so as to provide evidence for further research and clinical application of fat transplantation. Methods The differentiating 3T3-L1 cells after adipogenic induction were incubated with different concentration of S.miltiorrhiza (0.6 g/L, 0.3 g/L, 0.15 g/L, 0.075 g/L, 0.04 g/L, 0.02 g/L). After the 3T3-L1 cells differentiated into mature adipocyte, the cell proliferation viability was determined by CCK-8 assay kit. The lipid droplets accumulation of adipocyte were observed by Oil Red O staining and Image Pro Plus 6.0. The adipogenesis was quantified by measuring lipid content using triglyceride GPO-POD assay kit. Results S. miltiorrhiza promoted a dose-and time-dependent increase in 3T3-L1 cell proliferation viability. The earlier the dosing time was, the better the cell proliferation viability would be; The higher the concentration was, the better the cell proliferation viability would be. Within the six S.miltiorrhiza concentrations confine, S.miltiorrhiza also promoted a dose-dependent increase in 3T3-L1 cell differentiation ability, enhanced the lipid droplets accumulation of 3T3-L1 adipocyte. Conclusion S.miltiorrhiza can promote the increase of adipocyte number via the proliferation and differentiation of 3T3-L1 cells.