目的 探讨应用i RGD-外泌体-阿霉素抑制人恶性黑色素瘤细胞系A375体外增殖的有效性及其靶向性。方法体外转染i RGD,并提取外泌体,通过电穿孔包裹阿霉素,利用流式细胞仪检测i RGD-外泌体-阿霉素对A375细胞的亲和力,并对i RGD-外泌体-阿霉素抑制A375细胞的增殖能力进行分析。结果 i RGD-外泌体-阿霉素与A375细胞的结合率高于空白转染-外泌体-阿霉素;i RGD-外泌体-阿霉素可明显抑制A375细胞的增殖,空白转染-外泌体-阿霉素未观察到明显的抑制情况。结论 i RGD-外泌体-阿霉素能通过靶向作用于A375细胞,并抑制其增殖。

Objective To investigate the effect and targeting of iRGD-exosomes-doxorubicin (iRGD-exo-dox) on the inhibition of A375 cell line. Methods The exosomes were extracted from the iRGD-transfected A375 cells. The doxorubicin was wrapped by electroporation. Flow cytometry was used to detect the affinity of iRGD-exo-dox to A375 cells. The ability of iRGD-exo-dox on inhibiting the proliferation of A375 cells was analyzed. Results The affinity of iRGD-exo-dox to A375 cells was higher than the blank-exo-dox. The proliferation of A375 cells was obviously inhibited by iRGD-exo-dox. The inhibition effect was not observed in blank-exo-dox group. Conclusion iRGD-exo-dox can inhibit the proliferation of A375 cells by targeting function.