目的 探讨应用i RGD-外泌体-阿霉素抑制成纤维细胞体外增殖的有效性及靶向性。方法 体外转染i RGD,提取外泌体,通过电穿孔包裹阿霉素,利用流式细胞仪检测i RGD-外泌体-阿霉素对成纤维细胞的亲和力,并对i RGD-外泌体-阿霉素抑制成纤维细胞的增殖能力进行分析。结果 i RGD-外泌体-阿霉素对成纤维细胞的亲合力高于空白转染-外泌体-阿霉素;i RGD-外泌体-阿霉素可明显抑制成纤维细胞的增殖,空白转染-外泌体-阿霉素无明显的抑制作用。结论 i RGD-外泌体-阿霉素能通过靶向作用于成纤维细胞,以抑制成纤维细胞的增殖,可应用于瘢痕疙瘩的治疗研究。

Objective To investigate the effect and targeting of iRGD-exosomes-doxorubicin (iRGD-exo-dox) on the inhibition of the proliferation of fibroblasts. Methods The exosomes were extracted from the iRGD-transfected fibroblasts. The doxorubicin was wrapped by electroporation. Flow cytometry was used to detect the affinity of iRGD-exo-dox to fibroblasts. The ability of iRGD-exo-dox on inhibiting the proliferation of fibroblasts was analyzed. Results The affinity of iRGD-exo-dox to fibroblasts was higher than the blank-exo-dox. The proliferation of fibroblasts was obviously inhibited by iRGD-exo-dox. The inhibition effect was not observed in blank-exo-dox group. Conclusion iRGD-exo-dox can inhibit the proliferation of fibroblasts by targeting function, which can be used in the treatment of keloid.