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皮下预制膀胱脱细胞基质-丝素蛋白双层支架修复大鼠膀胱的实验研究

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  • 上海交通大学医学院附属第九人民医院泌尿外科;上海交通大学医学院附属仁济医院泌尿外科

网络出版日期: 2020-07-23

A Study on the Repair of Bladder by Pro-Producing Bladder AceHular Matrix Graft-Silk Fibroin in Rats

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  • Department of Urology, Shanghai Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine;Department of Urology and Andrology, Shanghai Renji Hospital, Shanghai Jiaotong University School of Medicine;

Online published: 2020-07-23

摘要

目的观察膀胱脱细胞基质-丝素蛋白(Bladder acellular matrix graft-silk fibroin,BAMG-SF)双层支架经皮下预制处理后,修复大鼠膀胱扩大术后膀胱缺损的效果。方法取BAMG-SF置于大鼠皮下,分别经1 d、3 d、7 d和14 d后,行组织学染色,评价其体内情况;实验分4组:单纯BAMG-SF组,BAMG-SF皮下预制3 d组、预制7 d组、预制自体组织组(BAMG-SF皮下预制7 d后去除BAMG-SF,保留周围结缔组织),分别修复大鼠膀胱扩大术模型,术后4周行HE、Masson及相关免疫组化染色,评价其修复情况。结果皮下预制7 d,即有大量细胞长入;皮下预制14 d,材料可被自身组织包裹;皮下预制3 d组的血管及肌肉修复程度均优于其他组,自体组织组未见结石形成。结论 BAMG-SF双层支架材料可用于膀胱缺损修复,皮下预制3 d组织修复效果最好,而自体组织组可以避免结石形成。

本文引用格式

王琼,肖冬冬,赵阳,周哲,周娟,王忠,张明,卢慕峻 . 皮下预制膀胱脱细胞基质-丝素蛋白双层支架修复大鼠膀胱的实验研究[J]. 组织工程与重建外科杂志, 2016 , 12(6) : 335 -339 . DOI: 10.3969/j.issn.1673-0364.2016.06.002

Abstract

Objective To observe the role of subcutaneous pretreated bladder acellular matrix graft-silk fibroin (BAMG-SF) for bladder reconstruction in a rat bladder augmentation model. Methods After BAMG-SF was implanted subcutaneously for 1 day, 3 days, 7 days and 14 days in 4 four-week-old female Sprague Dawley rat, the scaffold and tissues around it were harvested for histology evaluation. Set 4 groups (n=3): simple BAMG-SF (control group), BAMG-SF 3 days subcutaneous precondition (3 days group), BAMG-SF 7 days subcutaneous precondition (7 days group) and the autologous tissue around BAMG-SF 7 days subcutaneous precondition (autologous group). All groups were used to reconstruct bladder in a rat bladder augmentation model, the bladder was harvested for histology and immunology evaluation 4 weeks later. Results After BAMG-SF implanted subcutaneously for 7 days, a large number of cells were observed in BAMG-SF. After BAMG-SF implanted subcutaneously for 14 days, BAMG-SF was covered by autologous tissue. The reconstruction of vascular and muscle in 3 days group were better than other groups, only the autologous group had no stone found. Conclusion BAMG- SF can be used for bladder repair, 3-day group has the best effect on bladder reconstruction and autologous group can avoid stone formation effectively.
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