目的 使用叠层自组装技术在牙根面构建外源性蛋白缓释系统。方法 收集离体下颌第二前磨牙18颗,随机分为6组,a-e组为实验组,进行酸蚀处理;f组为对照组,不作酸蚀处理。各组在不同条件下进行叠层自组装实验,于第1~9天收集样本液,检测吸光度并计算蛋白释放浓度。结果 各组样本蛋白均有持续性释放,各组内均呈现缓慢释放的趋势(P<0.05)。酸蚀处理组蛋白释放量显著高于对照(P<0.05);随着原料中蛋白浓度增加,其释放量及缓释速率提高(P<0.05):随吸附次数的增加,蛋白释放量也提高(P<0.05),缓释速率基本不变,但释放维持时间延长。结论 叠层自组装技术能有效地在根面构建外源性蛋白的缓释系统。

Objective To construct a sustained release system of exogenous protein on the root surface using layer-by layer self-assembly coating technique. Methods Eighteen healthy mandibular second premolars were collected and randomly divided into 6 groups, group a-e were experimental groups with acid etching, group f was a control group without etching. Each group went through layer-by-layer adsorption experiments under different conditions. Samples were collected and detected absorbance on day 1-9 in each group to calculate the concentration of protein release. Results All sets of samples showed sustained-release trend （P〈0.05）. Etching group showed a significant increase of protein release compared with control group （P〈O.05）; Release rate and amount increased with the protein concentration in the materials （P〈0.05）; More adsorption times leaded to larger release amount and prolonged time （P〈0.05）. Conclusion Sustained release system of exogenous protein can be constructed on the root surface using layer-by layer self-assembly coating technique.