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软骨形态发生蛋白1诱导的瘢痕成纤维细胞体内成软骨能力的实验研究

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  • 上海交通大学医学院附属第九人民医院整复外科

网络出版日期: 2020-07-23

Chondrogenic Differentiation of Hypertrophic Scar -Derived Fibroblasts Initiated by Cartilage -derived Morphogenetic Protein 1 in Vivo

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  • Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine;

Online published: 2020-07-23

摘要

目的探讨软骨形态发生蛋白1(CDMP1)诱导的瘢痕成纤维细胞在体内环境下的软骨构建能力。方法取瘢痕切除术后丢弃的增生性瘢痕组织,提取瘢痕成纤维细胞。将瘢痕成纤维细胞与PGA/PLA支架复合,CDMP1软骨诱导液(CDMP1终浓度为100 ng/m L)进行诱导培养2周,设为诱导组(n=10);将常规培养液培养的瘢痕成纤维细胞-材料复合物植入裸鼠体内作为阴性对照,设为非诱导组(n=4);将软骨细胞-材料复合物植入裸鼠体内作为阳性对照,设为软骨组(n=4)。分别于4周和8周后取材,进行各组湿重、糖胺聚糖(GAG)含量测定,HE染色、Safranine-O染色和Ⅱ型胶原免疫组化染色。结果体内培养4周、8周后各组湿重、GAG含量测定显示,诱导组均高于非诱导组(P<0.05)。体内培养4周后,诱导组HE染色结果显示,瘢痕成纤维细胞诱导后出现软骨细胞陷窝结构;Safranine-O染色结果示,GAG均匀分布于基质;免疫组织化学染色示部分瘢痕成纤维细胞基质中COLⅡ阳性表达。8周时,诱导组的类软骨结果相对4周时更加成熟,更加符合软骨结构分布。结论在CDMP1诱导下,瘢痕成纤维细胞与PGA/PLA材料复合,在体内可以形成类软骨组织,具备一定的成软骨能力。

本文引用格式

沈聪聪,柴岗,曲淼,侯亦康,许祐荣,张艳 . 软骨形态发生蛋白1诱导的瘢痕成纤维细胞体内成软骨能力的实验研究[J]. 组织工程与重建外科杂志, 2014 , 10(6) : 324 -328 . DOI: 10.3969/j.issn.1673-0364.2014.06.006

Abstract

Objective To explore the chondrogenesis potential of hypertrophic scar fibroblasts (HSFBs) induced by cartilage-derived morphogenetic protein 1 (CDMP1) in vivo. Methods Hypertrophic scar fibroblasts (HSFBs) were isolated from discarded hypertrophic scar. HSFBs combining with PGA/PLA scaffold as HSFBs- PGA/PLA complex were induced by CDMP1 (100 ng/mL) for 2 weeks. Then the complex were transplanted into nude mice as induced group (n=10). The complex that not induced were treated as negative control, named as un-induced group ( n=4), while the chondrocytes-PGA/PLA complex were treated as positive control, named as chondrocyte group (n=4). After 4 and 8 weeks, the complex in each group was harvested for wet weight test, proteoglycan (GAG) content test, HE staining, Safranine-O staining and immunochemistry staining. Results After 4 and 8 weeks in vivo, the wet weight and GAG content in induced group were both higher than in un-induced group (P〈0.05). In induced group, after cultured for 4 weeks in vivo, chondrocyte-like lacuna structure was observed by HE staining, uniform distribution of GAG was observed by Safranine-O staining, and positive expression of collagen type Ⅱ was revealed by immunohistochemical staining. After 8 weeks of culturing in vivo, chondrocyte-like structure in induced group was more mature and more in line with the structure distribution of cartilage. Conclusion HSFBs combining with PGA/PLA had the potential to develop into polygonal chondrocyte-like tissue by the induction of CDMP 1.
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