组织工程与重建外科杂志

组织工程与重建外科杂志 >

2009 , Vol. 5 >Issue 1: 4 - 48

DOI: https://doi.org/10.3969/j.issn.1673-0364.2009.01.002

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长期体外培养对人脐血间充质干细胞成骨分化潜能的影响

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  • 上海组织工研究与开发中心;上海交通大学附属第九人民医院组织工程实验室

网络出版日期: 2020-07-23

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The Effects of Long-term Culture In Vitro on the Osteogenic Differentiation Potential of Human Umbilical Cord Blood Derived Mesenchymal Stem Cells

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  • 1 Shanghai Tissue Engineering Research and Development Center;Shanghai 200235;China. 2 Tissue Engineering Key Laboratory;Shanghai Ninth People′s Hospital;Shanghai Jiaotong University School of Medicine;Shanghai 200011;China.;

Online published: 2020-07-23

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摘要

目的观察体外长期培养对人脐血间充质干细胞(Human umbilical cord blood derived mesenchymal stem cells,UCB-MSCs)成骨分化潜能的影响,探讨其作为组织工程骨种子细胞的可行性。方法流式细胞技术观察长期体外培养的UCB-MSCs细胞表面抗原表达的变化规律,并通过Alizarin Red染色及碱性磷酸酶活性、骨钙蛋白含量和钙离子含量的检测观察长期体外培养对UCB-MSCs成骨特性的影响。结果第10代之前的UCB-MSCs能够高表达间充质干细胞表面标志物,7代之前的细胞体外增殖能力不随传代次数而发生明显变化。Alizarin Red染色及碱性磷酸酶活性、骨钙蛋白含量和钙离子含量的检测显示8代之前的UCB-MSCs仍能保持较强的成骨分化能力。结论7代之前的UCB-MSCs能保持稳定的体外成骨分化特性,有望成为较理想的组织工程骨种子细胞。

关键词： 人脐血间充质干细胞; 组织工程; 成骨分化; 体外培养

本文引用格式

刘广鹏，张文杰，李宇琳，孙剑，周恒，崔磊 . 长期体外培养对人脐血间充质干细胞成骨分化潜能的影响[J]. 组织工程与重建外科杂志, 2009 , 5(1) : 4 -48 . DOI: 10.3969/j.issn.1673-0364.2009.01.002

Abstract

Objective To observe the effects of long-term culture in vitro on the osteogenic differentiation potential of human umbilical cord blood derived mesenchymal stem cells (UCB-MSCs). Methods The CD markers of UCB-MSCs were identified by flow cytometric analysis. Osteogenic differentiation of UCB -MSCs at different passages was evaluated by Alizarin Red staining, and measurement of alkaline phosphatase activity, osteocalcin content, and calcium content respectively. Results The CD marker expression of UCB-MSCs ...

Key words： Human umbilical cord blood derived mesenchymal stem cells; Tissue engineering; Osteogenic differentiation; Long-term culture in vitro

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