目的评价不同浓度配比的壳聚糖-胶原支架材料对细胞亲和性的影响,并观察在细胞-支架复合物培养过程中支架材料的物理性质变化情况。方法采用第3代正常人皮肤成纤维细胞作为种子细胞,进行体外培养和扩增至足够数量后,分别种植于100%胶原(实验组1)、70%胶原(壳聚糖∶胶原=3∶7,实验组2)、50%胶原(壳聚糖∶胶原=5∶5,实验组3)、30%胶原(壳聚糖∶胶原=7∶3,实验组4)四种不同浓度配比的壳聚糖-胶原复合支架材料上,培养7d,通过光镜、电镜等观察和比较细胞在支架上的黏附情况、增殖活力和生长形态,以及材料物理强度的变化情况。结果不同浓度的各组壳聚糖-胶原支架材料上,细胞均能黏附、生长良好,其中实验组2的多孔支架材料上细胞增殖更接近单纯胶原材料(实验组1),且材料收缩降解速度明显低于其他2组。结论不同浓度配比的壳聚糖-胶原支架材料中,壳聚糖∶胶原=3∶7的复合支架材料具有良好的三维空间结构和细胞相容性,且材料形变相对较小。

Objective To investigate the influence of chitosan-collagen compound with different ratio for cell affinity and physical variation of the scaffold. Methods Human dermal fibroblasts of passage 3 were cultured and amplified in vitro, then seeded on the those scaffolds in different mixed ratio groups as follows: 100% collagen as group 1, 70% collagen (chitosan-collagen mixed with 3∶7) as group 2, 50% collagen (chitosan-collagen mixed with 5∶5) as group 3, 30% collagen (chitosan-collagen mixed with 7∶3) as grou...