目的研究A型肉毒素(BTA)对皮肤神经末梢释放的神经肽SP、CGRP的影响及其对皮肤创伤愈合的意义。方法40只SD大鼠随机分为C组(空白对照组)和E组(肉毒素注射组),20只/组。肉毒素A预注射后7d,在鼠背部建立以4个注射点为中心的创面模型,面积1cm×1cm。测量术后3d和7d的创面面积。实时定量PCR(Real-timePCR)检测BTA注射后第7天(术前)和创面愈合后(术后14d)的皮肤组织中感觉神经肽P物质(SP)、降钙素基因相关肽(CGRP)以及创伤愈合中的重要分子TGF-β1和α-SMA的mRNA表达。结合免疫组化和图像分析技术检测切片中上述4种物质阳性染色的积分光密度OD值。结果①各组创面于术后14d基本愈合。与C组相比,E组术后3d、7d的创面面积无差异(P>0.05);②E组术前和术后14d的皮肤组织中SP、CGRP、TGF-β1和α-SMA的mRNA表达和OD值较C组明显降低,差异有统计学意义(P<0.05)。结论①局部皮下注射BTA不影响创面愈合的时间和速度;②BTA能减少创面愈合过程中皮肤神经末梢对SP、CGRP的释放和创面愈合过程中TGF-β1和α-SMA的表达。

Objective To investigate the effect of botulinum toxin type A on the expression of substance P and calcitionin gene-related peptide in wound healing. Methods Forty rats were randomly divided into Group C (Control) and Group E (Experimental), 20 rats in each group. The wound-healing model was established by excised four full-thickness skin (1 cm × 1 cm, circled the injection site) in the back of all SD rats 7 days after BTA injection. The area of wound was measured and calculated at the 3rd d and 7th day aft...