组织工程与重建外科杂志

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2009 , Vol. 5 >Issue 6: 310 - 313

DOI: https://doi.org/10.3969/j.issn.1673-0364.2009.006.003

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人脐带间充质干细胞向血管内皮祖细胞诱导分化的实验研究

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  • 上海交通大学医学院附属上海儿童医学中心胸心外科; 上海交通大学医学院附属第一人民医院整形外科; 上海交通大学医学院附属上海儿童医学中心骨科

网络出版日期: 2020-07-23

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Experimental Study of Human Umbilical Cord Mesenchymal Stem Cells on Differentiation of Vascular Endothelial Progenitor Cells in Vitro

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  • 1 Department of Cardio-Thoracic Surgery; Shanghai Children’s Medical Center Affiliated Shanghai Jiaotong University School of Medicine; Shanghai 200127; China; 2 Department of Plastic Surgery; First People’s Hospital of Shanghai Jiaotong University School of Medicine; Shanghai 200080; China; 3 Department of Orthopaedics; Shanghai Children’s Medical Center Affiliated Shanghai Jiaotong University School of Medicine; Shanghai 200127; China.

Online published: 2020-07-23

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摘要

目的探讨人脐带间充质干细胞定向诱导分化为血管内皮祖细胞的可行性,为构建组织工程血管瓣膜内皮化提供新的细胞来源。方法无菌条件下取剖宫产新生儿脐带,复合胶原酶消化法获取脐带间充质干细胞进行培养,以流式细胞仪检测脐带间充质细胞的表面标志。取扩增3～6代的脐带间充质干细胞用VEGF和b-FGF诱导分化。用免疫荧光法鉴定内皮祖细胞的标志。结果脐带间充质干细胞可以诱导分化为血管内皮样细胞,表达CD34、CD133和vWF,且Dil-acLDL实验阳性。结论脐带间充质干细胞可以诱导分化为血管内皮祖细胞,可为构建组织工程血管瓣膜提供新的种子细胞来源。

关键词： 脐带间充质干细胞; 血管内皮细胞; 诱导分化; 组织工程

本文引用格式

陈恩，祝加学，秦金保，丁文祥，俞晓青，高波涛 . 人脐带间充质干细胞向血管内皮祖细胞诱导分化的实验研究[J]. 组织工程与重建外科杂志, 2009 , 5(6) : 310 -313 . DOI: 10.3969/j.issn.1673-0364.2009.006.003

Abstract

Objective To explore the differentiation of human umbilical cord mesenchymal stem cells(UMSCs).Methods The UCMSCs were isolated from full-term neonates.MSCs were obtained by mixed enzymatic digestion and cultured in DMEM/10%FBS.Surface antigens of UCMSCs were detected by flow cytometry.Different passages of MSCs(P3-P6) were induced to differentiate towards endothelial progenitor cells by VEGF and b-FGF,then endothelial cells markers were detected by immunofluorescence.Results Human umbilical cord mesenchymal stem cells can be induced to endothelial cells lineage.Immunofluorescent assay showed that different passages of MSCs could express CD34,CD133 and vWF and Dil-acLDL assay was positive.Conclusion Human umbilical cord mesenchymal stem cells could be induced into vascular endothelial cells,which could be used as a new source of seeding cells for tissue engineered blood vessel and cardiac valve.

Key words： Human umbilical cord mesenchymal stem cells; Vascular endothelial progenitor cells; Differentiation; Tissue engineering

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