DREB has an important role in stress signal transduction pathways in plants.A cDNA fragment, named SmDREB3, was isolated from ‘YZ-14’ eggplant species using homology-based cloning method.The full length of cDNA of SmDREB is 887 bp, and the ORF is 777 bp, encoding 259 amino acids.It had 90% homologies with the sequence of potato and 88% homologies with the sequence of tomato. The mature protein isoelectric point was at pH 6.39 and molecular weight was 28.665 kDa.RT-qPCR analysis indicated that SmDREB3 was expressed in all organs.However, the expression level of SmDREB3 was tissue-specific.It expressed highest in the pericarp, followed by the leaf and sarcocarp, while it expressed relatively low in roots, stems and flowers.This study provides the theoretical basis for further researches on resilience mechanisms and cultivating new varieties that resist adverse environment.
Stockinger E J, Gilmour S J, Thomashow M F.Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit[J].Proceedings of the National Academy of Sciences, 1997, 94(3):1035-1040.
doi: 10.1136/bjo.85.10.1248
Matsukura S, Mizoi J, Yoshida T, et al.Comprehensive analysis of rice DREB2-type genes that encode transcription factors involved in the expression of abiotic stress-responsive genes[J].Molecular Genetics and Genomics, 2010, 283(2):185-196.
doi: 10.1007/s00438-009-0506-y
pmid: 20049613
Chen M, Xu Z, Xia L, et al.Cold-induced modulation and functional analyses of the DRE-binding transcription factor gene, GmDREB3, in soybean(Glycine max L.)[J].Journal of experimental botany, 2009, 60(1):121-135.
doi: 10.1093/jxb/ern269
pmid: 3071762
[8]
Islam M S, Wang M H.Expression of dehydration responsive element-binding protein-3(DREB3)under different abiotic stresses in tomato[J].BMB Reports, 2009, 42(9):611-616.
doi: 10.5483/BMBRep.2009.42.9.611
pmid: 19788864
[9]
Behnam B, Kikuchi A, Celebi-Toprak F, et al.Arabidopsis rd29A::DREB1A enhances freezing tolerance in transgenic potato[J].Plant Cell Reports, 2007, 26(8):1275-1282.
doi: 10.1007/s00299-007-0360-5
