Dichlorvos Degradation by REMI Mutants of Trichoderma koningii
Xu YUAN1,2, Li-ping JIAN3, Jie CHEN1,2()
1.School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China 2.Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai 200240, China 3.School of Plant Protection, Shenyang Agricultural University, Shenyang 110161, China
The dichlorvos degradation abilities of Trichoderma koningii mutants obtained by REMI (restriction enzyme mediated integration) and the optimal conditions for effective degradation were studied.Results showed both the wild type (T30) and mutants (TK-1, TK-2, TK-3, TK-5, TK-7, TK-8, TK-21, TK-38, TK-53, TK-30, TK-42)were able to degrade dichlorvos.TK-3 could reach 98% dichlorvos decomposition, which is the most efficient among mutants.Dichlorvos degradation rate was closely associated with glucose, dichlorvos initial concentration and pH of the medium.It was found that glucose at 1 000 μg/mL, pH 7.0 and dichlorvos at 500 μg/mL were the optimal conditions for effectively degrading dichlorvos by the mutant TK-3.The degradation efficiency of TK-3 would decline if dichlorvos concentration exceeded 1 000 μg/mL.
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