1 Introduction
The report of 19th National Congress states that “A beautiful China needs a clean and low-carbon energy system”. The goals of international energy security and sustainable development necessitate the role of renewable energy, of which biomass energy is an essential choice for realizing the strategic energy diversification. Microbial conversion of flue-gas-derived CO2 for producing biodiesel and biogas has been considered a significant technology in many industries, such as energy conservation, environmental protection, new energy development, and low-carbon circular economy. The Outline of the National Program for Long- and Medium-Term Scientific and Technological Development and the 13th Five-Year Plan For Renewable Energy Development clearly point out that it is necessary to vigorously develop new energy, such as biomass energy and hydrogen energy, to achieve the targets in which 2 million tons of biodiesel and 44 billion cubic meters of biogas should be produced by 2020. Prestigious peer-review journals such as Nature and Cell have pointed out that microbial energy is one of the hottest research frontiers. However, there are still three basic problems in energy/mass transfer and conversion in the process of microbial conversion of flue-gas-derived CO2 to biodiesel and biogas fuels, the first is the mass transfer of CO2 across cell membrane, the research of Prof. Ned Wingreen showed that the growth rate and CO2 fixation efficiency of microalgae under air conditions were seriously limited by CO2 diffusion rate [1], Khoo et al. found that the contradictory relationship between high resistance of cell membrane micropores and high flux of flue-gas-derived CO2 limited mass transfer rate of CO2 molecules across cell membrane [2]. The second is the intracellular enzyme activity of microalgae. Prof. Martin jonikas has proved that the low biological catalytic activity of enzymes in cells with high concentration of CO2 leads to the difficulty of directional carbon/hydrogen conversion [3], how to improve the activity of key enzymes is still a key problem in the process of microalgae carbon sequestration [4]. The third is problem of competitive intracellular reaction pathways. Many researchers have found that nitrogen starvation was able to promote the synthesis and accumulation of oil in microalgae cells, but reduce the photosynthetic activity of microalgae cells. There is a conflict between CO2 fixation and lipid accumulation [5,6]. The competition between multiple intracellular reaction pathways and high energy barriers of target products hinder the desirable cascade energy transfer. Prof. Heinz kopetz proposed that the catalytic reaction of direct conversion of carbon dioxide to lipids is the key to the production of lipids by microalgae [7]. Therefore, key scientific issues of microbial energy conversion lie in the understanding on directional carbon conversion and desirable cascade energy transfer. Facing to those key problems, multiple researcheres haveconducted a long-term research on the topic of microbial conversion of flue-gas-derived CO2 for producing biodiesel and biogas, which established a theoretical foundation of microbial energy conversion and broke the bottlenecks of strengthening energy/mass transfer in microbial cells. Through those studies, they have revealed the mass transfer mechanism of vortex flow across cell membrane micropores, proposed a strategy that directionally regulated enzyme activity, and established the chain reaction pathways coupled with step changes. Therefore, a series of innovative results through long-term research have been obtained as follows:
1. Mass transfer mechanism of vortex flow across cell membrane micropores has been revealed. It was comprehensively discussed that the scientific problem of limited mass transfer at the microporous interface of cell membrane from the perspective of photon conversion during photosynthesis [8,9,10]. The mechanism explained the behaviors of hydrodynamic mass transfer in terms of vortex enhanced intracellular photon transformation, and further solved the problem of low CO2 transport rate across the micropore-interface of cell membrane.
2. A strategy that directionally regulates enzyme activity has been proposed. A scientific issue of improving biocatalytic activity from the electron transfer perspective has been issued and the approach coupling gradient flue-gas-derived CO2 domestication with mutagenesis of enzymes to enhance electron transfer was proposed [10]. Besides, they also developed an approach to regulate active sites of CO2 fixation enzymes, thus significantly improving the key enzyme activity [11].
3. A chain reaction pathways coupled with step changes has been established. It was identified that high energy barrier was the obstacle of cascade energy transfer, and chemical chain reaction model combining the functions of CO2 fixation enzymes and lipid synthesis enzymes wasestablished [12]. The energy barrier step pathways of C1→C6→C18 chain elongation was revealed, thus developing principles of cascade energy transfer to solve trade-off relationship between CO2-biofixation and lipid-synthesis [13,14].
2 Research progress on strengthening energy and mass transfer in microbial conversion of flue-gas-derived CO2 to biodiesel and biogas fuels
As shown in Figs. 1, 3 main problems in microalgal research limited the CO2 conversion to biogas and biofuels, including the mass transfer rate limitation, low biocatalytic activity of intracellular energy for directional carbon conversion and high energy barriers of target products. Therefore, we planned to make a breakthrough in 3 aspects including CO2 transport, directional carbon conversion and lipid/gas products.
Fig. 1 Key scientific challenges of biodiesel and biogas production based on microbial conversion of flue-gas-derived CO2 |
2.1 A method of high CO2 flux using flue gas with vortex formation
In the context of the huge emissions of flue gas from coal-fired power plants and the high CO2 content (up to 15%), fixation and conversion of flue-gas-derived CO2 to gas and oil fuels using microalgae has been a research hotspot in the research field of energy. Wingreen et al. [1] proved that the growth rate and CO2 fixation efficiency of microalgae under air conditions are seriously limited by CO2 diffusion rate. Due to the complex process: (1) the low concentration (400 ppm) of CO2 molecules (2.4 Å) must first be dissolved in water as the inorganic carbon form of HCO3− (3.1 Å); (2) then HCO3− combined with transport proteins go across cell membrane into cell; (3) finally CO2 will be released with the catalysis of carbonic anhydrase. This theory has been widely accepted by researchers in this area, but only limited advances have been achieved in terms of improving the overall reaction rate of microalgal CO2 fixation. In recent years, many researchers began to explore the scientific problem of limited mass transfer at the microporous interface of cell membrane from the perspective of photon conversion in photosynthetic process. Hu et al. found that making full use of microalgae flash effect, making algal cells periodically exposed to light/dark conditions was able to effectively promote microalgae growth [15]. Due to the different types of reactors, microalgae varieties and breeding environment, there is no unified understanding of the optimal flash frequency of microalgae photosynthetic reactor, but they all agree that the flash effect can effectively improve the growth rate of microalgae [16]. Some scholars have studied the effect of flash frequency on the growth of microalgae at the millisecond level [17], however, most of these studies were carried out in the laboratory by changing the artificial light frequency or periodically shielding some reactors [18,19]. In the natural state, realizing the millisecond flash cycle need strong mixing flow, which will greatly increase the breeding system in energy consumption. A case study on comparison of life cycle analyses of microalgal biomass production has been reported by Prof. Jorquera [20]. The authors have reported the net energy ratio (NER) for both the processes for microalgae production, e.g. tubular and flat bed reactors as well as raceway ponds. The NER of a system has been defined as the ratio of the total energy produced (energy content of the oil and residual biomass) over the energy content of photobioreactor construction and material plus the energy required for all plant operations: NER = NetEnergyRatio = ΣEnergyproduced(lipidorbiomass)∑Energyrequirements. The results indicate that the use of horizontal tubular photobioreactors (PBRs) is not economically feasible due to negative NER values. The NER values for flat bed PBRs and raceway ponds are found to be positive. The research of Dasan et al. [21] shows that at present, the dehydration and oil extraction of microalgae biomass require high energy input, accounting for nearly 21% - 30% and 39% - 57% of the total energy demand respectively. The research of Morweiser et al. [22] shows that in terms of energy efficiency, the best reactor (such as different ‘water bed’ designs) has now entered the range of 50% auxiliary energy demand related to the collected solar energy (or 2 w/m2 and 0.04 m3/m2) by reducing the auxiliary energy to 50 w/m3.
Therefore, more advanced photobioreactors and low-cost downstream processing technologies were sought to achieve a more feasible microalgae biofuels production system [23]. Based on continuous illumination conditions, the photosynthetic reactor with second flash cycles through turbulence to enhance the turbulent flow of culture medium is more suitable for industrial scale aquaculture. Zhang et al. [24] developed a spiral static mixer, which accelerated the mixing and light dark cycle of the column photoreactor, thereby increasing the biomass yield by 37.26%. Chen et al. [25] introduced six half moon blade static mixers into the column photoreactor and found that the mixer improved the turbulent kinetic energy and light dark cycle frequency. Zhao et al. [26] proposed a multi-scale bubble combined inlet method to strengthen the multiphase mixing and mass transfer process of Chlorella by using millimeter bubbles with strong turbulence characteristics. With large contact area and long residence time, microbubbles can significantly improve the gas retention rate, light utilization efficiency and growth rate of small particle microalgae in microalgae photosynthetic reactor. Ye et al. [8] used the nozzle to generate vortex to enhance the photochemical efficiency of microalgae photosynthetic column reactor. However, due to the small vortex radius and mainly concentrated at the bottom of the reactor, most areas in the upper part of the reactor are little affected by the vortex.
The photobioreactor designed by Prof. Cheng of Zhejiang University based on the theory of hydrodynamics produced alternating clockwise and counterclockwise vortex flow fields, strengthened the flash effect of rapid flow of algae cells in light and dark areas (Fig. 2), increased the gas-liquid two-phase mass transfer coefficient by 25%, and increased the biomass yield by 32.6% [9]. Aiming at the new problems about flow and mass transfer with microbial photosynthesis, they revealed the phenomenon that vortex strengthens the photosynthesis process of microalgal cells [27]. Prof. Dubinsky evaluated that “the novel system” proposed in this research “sequentially generate clockwise and anticlockwise liquid vortexes”, “increased the mass transfer coefficient”, and “enhanced flashing-light effect” [16]. Professor Simon Judd evaluated this finding and believed that ‘HD removal rate was achievable’ [28]. Professor Cheng Jun’s team of Zhejiang University proposed to use a ground-breaking mechanism of “ flashing light effect of vortex flow”, which advanced the conventional understanding on transmembrane transport of CO2 molecules with HCO3− as the intermediate, so as to reveal the hydrodynamic mass transfer mechanism of vortex, thereby enhancing intracellular photon conversion efficiency [10].
Fig. 2 High flue-gas-derived CO2 flux with generation of vortex flow to promote CO2 transfer across microalgal cell membrane and cellular photon conversion efficiency |
2.2 A flue-gas-derived CO2 domestication strategy that directionally regulates enzyme activity
The CO2 concentration of 15% in flue gas from coal-fired power plant is about 300 times the atmospheric CO2 concentration. Although high CO2 concentration from flue gas improves diffusion and transfer of CO2 molecules across cell membrane of microalgae, the biological catalytic activity of key CO2 fixation enzymes in original microalgal cells is severely suppressed under such a high CO2 concentration condition. Thus, how to efficiently improve the activity of key CO2 fixation enzymes in microalgal cells is an international research hotspot. Martin Jonikas [3] proved in that traditional biological enzymes have low catalytic activity in the process of microbial energy conversion, resulting in difficulties in directional carbon/hydrogen conversion. Therefore, how to improve the activity of key enzymes from the perspective of quality/energy transfer of basic reactions in multiple cells is still a bottleneck in the process of microbial biomass hydrogen production. Xu et al. [29] established a model to help understand the changes of cell fluid flux, volume and media molar concentration caused by osmotic pressure. Assuming that cells were submerged in the CPAs, the water flux across the cell membrane (Jw) was given as.
$ {\mathrm{J}}_{\mathrm{W}}={\mathrm{L}}_{\mathrm{p}}\triangle \mathrm{P}\hbox{-} {\mathrm{L}}_{\mathrm{p}}{\sigma}_{\mathrm{s}}\mathrm{RT}\triangle \mathrm{c}, $
$ {\mathrm{P}}^i-{\mathrm{P}}^e=\mathrm{E}\frac{V-{V}_0}{V_0}+{P}_0^i-{P}_0^e $
$ \frac{dV_w}{dt}={J}_wA $
where Lp (m3 N− 1 s− 1) indicated the membrane hydraulic conductivity; σs was the membrane reflection coefficient of media; R (J mol− 1 K− 1) was the universal gas constant; T (K) was the temperature; c (M) was the concentration of media; A (m2) was the cell surface area; E (Pa) was the cellular elastic modulus; and Vw (m3) was the water volume of cells. In addition, superscripts i and e in the above equations denoted the intracellular and extracellular regions, respectively. Next, the media flux (Jc) was expressed as
$ {\mathrm{J}}_c=\left(1-{\sigma}_s\right){c}_{up}{J}_w+\omega RT\Delta c $
$ \frac{dV_c}{dt}={J}_cA $
where ω (mol N− 1 s− 1) and cup (M) were the membrane permeability of media and the upstream concentration of media, respectively. Huang et al. [14] found that when a higher concentration of CO2 was continuously injected into the aqueous solution for microalgae growth, the dissolved CO2 concentration in the solution increased significantly. Transcriptome sequencing showed that carbonic anhydrase was almost not expressed at this time, indicating that CO2 directly penetrated into microalgae cells by osmotic pressure to participate in Calvin cycle reaction. This process reduces the active transport of HCO3−, saves more energy and improves the efficiency of photosynthesis. The results showed that the cultivation of microalgae with high concentration of CO2 was able to promote the growth, carbon sequestration rate and biomass yield of microalgae [30].
Hussain et al. [31] found that the two microalgae plants growth was slow under high CO2 concentration (20% CO2). However, in step wise CO2 feeding, the growth of microalgae improved considerably and up to 0.9 and 0.97 (g/L) biomasses were recorded, respectively. According to the professor Ghosh’s research results, Scenedesmus sp. could effectively exploit high CO2 concentration (15%) for longer duration under high concentration of glucose supplementation (9 g/L) producing a biomass of 635.24 +/− 39.9 μg /mL with a high total fatty acid methyl ester (FAME) content of 71.29 +/− 4.2 μg /mg [32]. Yun et al. [33] during the growth of Chlorella vulgaris, the CO2 concentration in the gas gradually increased from 5% to 30% with the culture time (about every 43 h, the CO2 concentration increased by 5% or 10%), and the maximum CO2 fixation rate was 0.936 g / (L•D). Fulke et al. [34] in India screened a strain of Chlorella sp. from carbonate rich areas. After domestication with low carbon source and high temperature culture, the obtained Chlorella sp. can withstand 15% CO2 environment. Although some articles believe that overexpression of C4 gene in C3 plants cannot significantly improve the photosynthetic capacity of plants [35]. The research of Liu et al. [36] proposed that carbon fixation is carried out through the combination of C3 and C4 pathways and carbonic anhydrase, and the enhancement of C4 pathway by high CO2 concentration may provide a variety of carbon fixation pathways for C3 microalgae and finally improve its CO2 fixation capacity. Anjos et al. found that CO2 with volume fraction of 6% is most suitable for microalgae growth, but the volume fraction of CO2 in power plant flue gas is about 12% ~ 15%. Through nuclear mutation, screening and domestication, cultivate algae species resistant to high volume fraction CO2 and apply it directly to industrial aquaculture [37]. Nuclear mutagenesis induces recombination and improvement of key genes relating to CO2 fixation enzymes and lipid synthesis enzymes in microalgae cells, thus significantly enhancing the activities of CO2 fixation enzymes (e.g., photosynthetic pigment enzymes, ATP synthase, etc) and lipid synthesis enzymes (e.g., acetyl-CoA carboxylase and glycerol transferase) (as shown in Fig. 3). Vigeolas et al. [38] mutated Chlorella sorokiniana by ultraviolet radiation, screened 2000 single plants after mutation by Nile red fluorescence method, and successfully selected 4 Chlorella mutants with increased oil content. Tanadul et al. mutated Chlorella sp. by EMS, and the mutant obtained has higher biomass yield and oil yield [39].
Fig. 3 Enhancement of electron transfer through gradient flue-gas-derived CO2 domestication with gene mutagenesis of CO2 fixation enzymes |
Prof. Cheng Jun of Zhejiang University proposed a mutagenesis method that uses nuclear radiation to modify the genes relating to key enzymes for catalytic activity enhancement, thus promoting the energy and mass transfer with internal and external collaboration during electron transfer between key enzymes [11]. The research results of Prof. Cheng’s research group showed that the photosynthetic pigment enzymes and ATP synthase of the nuclear mutagenized strain increased by 6.8 times and 8.0 times, respectively, as compared to the conventional microalgal strains for CO2 fixation, thus simultaneously increasing the growth, CO2 fixation, and lipid accumulation rates of microalgal cells [12]. A 15-fold activity up-regulation of key CO2 fixation enzyme after gradient flue-gas-derived CO2 domestication was recorded [40]. This approach significantly improved the key enzyme activity and selectively enabled the promotion of elementary reactions of CO2 fixation towards efficient C3 pathway rather than inefficient C4 pathway, thus addressing the issue of low catalytic activity of conventional biological enzymes. Ren et al. [41] evaluated these studies and considered that a major breakthrough had been made in the transformation of microalgae strains to produce carbon dioxide and lipids by nuclear mutagenesis. Sachs and others can directionally promote lipid synthesis in microalgae cells by studying gene mutation to enhance the activity of lipid synthase [42].
2.3 Established chain reaction pathways coupling the reaction energy barrier step with chemical chain of enzymes to regulate competitive reaction pathways for biodiesel and biogas production
The ability of microalgae to fix CO2 and accumulate lipids depends on the growth rate and lipids content in microalgal cells. Because the energy density of lipids is twice as much as those of proteins and carbohydrates, the high energy barrier of direct conversion from CO2 to lipid causes low reaction rate. The research of Heinz Kopetz showd that the catalytic reactions of direct conversion from CO2 to lipids was key to lipid production in microalgae [7]. However, there is a conflict between microalgal CO2 fixation and lipid accumulation. Most microalgal species start to accumulate significant amounts of lipid under stress or unfavorable growth conditions [5]. Seo et al. [6] found that the lack of nitrogen salt in the solution would promote the synthesis and accumulation of oil in microalgae cells. However, nitrogen starvation significantly reduced the photosynthetic activities of algal cells and thus the overall productivity of algal biomass. Also, at the industrial level, nitrogen removal is time-consuming and costly [5]. Therefore, researchers seeking a versatile alternative method for practical induction of lipid accumulation remain active in the field of microalgal biotechnology [43]. Srivastava and Goud [44] investigated the effects of various salt stresses (NaCl, KCl, MgCl2 and CaCl2) on lipid accumulation in both Chlorella. sorokiniana CG12 and Desmodesmus GS12 strains, and obtained significant enhancements of lipid contents, up to 40-45% (w/w) under optimal CaCl2 conditions. Kang et al. [45] investigated the usefulness of oxidative stress by TiO2 nanoparticles, a well-known photocatalyst, to the induction of lipid accumulation in Chlorella Vulgaris UTEX 265. They observed a slight increase of Chlorella vulgaris under TiO2/UV-A conditions, though a high dosage of TiO2 (0.1 g/L) and 2-day incubation were required. Praveenkumar et al. [46] reported a new pressure-based stress method for induction of neutral lipid (e.g. TAG) of Chlorella spp. by 2 h treatment under mild pressurization conditions (10-15 bar). Compared with the untreated control, this method produced a 55% improvement.
CO2 is the only carbon source for photosynthetic autotrophic microalgae. When CO2 concentration increases, the content of fatty acids in microalgae will increase, and the content of polyunsaturated fatty acids in eukaryotic algae cells will decrease. This is because the increase of CO2 concentration will cause the excess of carbon source, which will cause the relative lack of nitrogen and phosphorus nutrients, thus affecting the synthesis of enzymes related to extension reaction and desaturation reaction. Cheng et al. found that the content of oil in a single cell increases when nitrogen and phosphorus are deficient, and the decrease of nitrogen and phosphorus nutrient content in the solution would affect the synthesis of enzymes related to prolongation reaction and desaturation reaction, and finally reduce the content of polyunsaturated fatty acids in the oil components of microalgae cells [12]. Yu et al. [47] evaluated the effect of plant hormone Gibberellin on the accumulation of lipid and docosahexaenoic acid (DHA) accumulation in Aurantiochytrium sp. YLH70. Metabolic pathway analysis showed that gibberellin accelerated the rate of utilization of glucose, and metabolites in fatty acids biosynthesis and mevalonate pathway were increased, while metabolites in glycolysis and TCA cycle were decreased in Aurantiochytrium sp. YLH70. Costa and Ge et al. also found that high concentration of CO2 is conducive to the synthesis of fatty acids in grape brown algae 765 and other algae species, and also inhibits the extension reaction and desaturation reaction of carbon chain [48,49]. The results of Tang et al. Showed that both Scenedesmus obliquus sjtu-3 and Chlorella pyrenoidosa sjtu-2 grew fastest at 10% CO2 concentration, while the oil content continued to increase when the CO2 concentration increased to 30-50% [50]. Table 1 summarizes the different induction methods for promoting microbial production of biodiesel and biogas fuel, and compares the induction effects.
Table 1 Comparison of different induction methods to promote microbial production of biodiesel and biogas fuel |
| Microalgal species | Induction method | Effects | Refs. |
|---|---|---|---|
| Green microalga Acutodesmus dimorphus | Nitrogen starvation | Promoted the biosynthesis of abscisic acid (ABA) and indole-3 acetic acid (IAA) | Chokshi et al. [51] (2017) |
| Chlorella spp. | Very short time mild pressure induction (10-15 bar). | The increase of cytoplasmic tag particles and rapid lipid remodeling/transformation | Praveenkumar et al. [46] (2016) |
| Chlorella sorokiniana CG12 and Desmodesmus GS12 | Salt stresses (NaCl, KCl, MgCl2和 CaCl2) | Increased lipid content, up to 40-45% (w/w) | Srivastava et al. [44] (2017) |
| Scenedesmus sp. LX1 | Two auxins (indole-3-acetic acid (IAA) and 2,4-dichlorophenoxyacetic acid (2,4-D) | Enhanced microalgal growth and FAMEs accumulation | Dao et al. [52] (2018) |
| Haematococcus pluvialis | Butylated hydroxytoluene (BHT), high-light and nitrogen-deficiency conditions | The astaxanthin and lipid contents of H. pluvialis were increased by 71.13% and 10.71% | Zhao et al. [53] (2018) |
| Aurantiochytrium sp. YLH70. | Phytohormone gibberellin | Metabolites in fatty acid biosynthesis and mevalonate pathway increased, metabolites in glycolysis and TCA cycle decreased | Yu et al. [47] (2016) |
The novel chain reaction pathways coupled with biocatalyst in microalgae are capable of reducing the energy barrier during direct conversion of CO2 to lipids. Prof. Cheng Jun of Zhejiang University analyzed CO2 fixation and lipid accumulation in microalgal cells from the view of energy barrier in intracellular REDOX reactions. Prof. Cheng established the chemical chain reaction model combining the functions of CO2 fixation enzymes and lipid synthesis enzymes, and broke the bottleneck of high energy barrier in the direct conversion of small molecular CO2 to high molecular lipids [13]. This leads to the development of the chemical chain reaction model (CO2 + C5 → 2C3↑ → C6H12O6 → (RCOO)2C3H6O↑ → (RCOO)3C3H5↑), which combines the functions of CO2 fixation enzymes and lipid synthesis enzymes. The model reveals the energy barrier step pathways of cell REDOX reactions, including the pathways of C1 → C6 → C18 chain elongation (Fig. 4). The research regulated the multiple intracellular competitive reactions of lipid synthesis, leading to the establishment of general principles for cascade energy transfer [12,14].
Fig. 4 Energy and mass transfer enhancement of lipid accumulation through chain reaction pathways coupling microalgal intracellular enzymes with step changes |
3 Conclusion
Through the research on the microbial conversion of flue gas CO2 into biodiesel and biogas, the mass transfer mechanism of vortex flow across cell membrane micropores was revealed, a strategy that directionally regulates enzyme activity was proposed, and the chain reaction pathways coupled with step changes was established. Those researches have served as the theoretical foundation for strengthening energy and mass transfer in microbial conversion of flue-gas-derived CO2 into oil and gas fuels, driven forward the development of engineering thermophysics, and made significant contributions to the development of biomass energy industry and the establishment of clean and low-carbon energy system.
Abbreviations
acetyl-CoA:Acetoacetyl coenzyme A;ATP:Adenosine triphosphate;C3:A CO2 fixation pathway that CO2 is directly fixed by five carbon compounds in Calvin cycle, and the initial product is three carbon compounds;C4:In addition to the Calvin cycle, CO2 combines with phosphoenolpyruvate in the dark reaction of photosynthesis, with oxaloacetic acid as the initial product;C6H12O6 :Glucose;CH3CH2CH2COOH:n-butyric acid;CH3CH2OH:Ethyl alcohol;CH3COOH:Acetic acid;CO2 :Carbon dioxide;H2 :Hydrogen;HCO3 − :Bicarbonate;IF:Impact Factor;SCI:Science Citation Index;TAG:Triglyceride
Acknowledgements
This research was supported by National key research and development program-China (2016YFB0601000) and National Key Research and Development Program-China (2017YFE0122800).
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Authors’Contributions
JC carried out conceptualization, methodology, formal analysis, writing and visualization. KC carried out conceptualization, writing - review & editing and supervision. The authors read and approved the final manuscript.
Funding
This research was supported by National key research and development program-China (2016YFB0601000) and National Key Research and Development Program-China (2017YFE0122800).
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