诊断学理论与实践 ›› 2017, Vol. 16 ›› Issue (03): 297-300.doi: 10.16150/j.1671-2870.2017.03.013

• 论著 • 上一篇    下一篇

ABO血型正反不符原因分析及输血策略

王钰箐, 蔡晓红, 龚淞颂, 王学锋, 邹纬   

  1. 上海交通大学医学院附属瑞金医院临床输血科,上海 200025
  • 收稿日期:2017-03-20 出版日期:2017-06-25 发布日期:2017-06-25
  • 通讯作者: 邹纬 E-mail: zouwei1998@sina.com

A study of cases with ABO typing discrepancy and the strategy of blood transfusion

WANG Yuqing, CAI Xiaohong, GONG Songsong, WANG Xuefeng, ZOU Wei   

  1. Department of Clinical Transfusion, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
  • Received:2017-03-20 Online:2017-06-25 Published:2017-06-25

摘要: 目的: 对ABO血型鉴定中正反不符的原因进行分析,探讨其输血策略。方法: 使用血清学方法鉴定ABO血型,对其中正反不符者采用ABO基因外显子6及7进行聚合酶链反应扩增并测序,确认ABO亚型。结果: 84 487例次ABO血型鉴定中共检出74例次ABO血型正反不符,不符率为0.09%。除去重复例次后共有67例ABO血型正反不符者,其中红细胞抗原减弱43例,血型抗体减弱9例,抗原及抗体同时减弱者2例,亚型12例,其他1例。36例ABO血型正反不符住院患者中,25例接受了输血治疗,22例为血液疾病患者。排除亚型病例后,统计输注ABO基因型同型红细胞15例,输注O型洗涤红细胞5例,采用t检验分析其输血效果,结果无统计差异(P=0.225)。结论: ABO血型正反不符者中血液疾病患者比例最高,临床输血结合血清学及分子生物学结果,可输注ABO相容或基因型同型红细胞。

关键词: ABO血型, 血型鉴定, 输血

Abstract: Objective: To analyze the cause of ABO typing discrepancy and to explore the strategy of blood transfusion. Methods: ABO blood group was identified by serological method, and the cases with ABO typing discrepancy were confirmed by molecular biological method. Results: Seventy-four cases of ABO typing discrepancy were detected in 84 487 cases, the discrepancy rate was 0.09%. Removing repeated cases, there were 67 cases: red blood cell antigen weakened 43 cases, blood group antibody weakened 9 cases, both antigen and antibody weakened 2 cases, subtypes 12 cases, others 1 case. In 36 hospitalized patients of ABO typing discrepancy, 25 cases received blood transfusion, and 22 were patients with hematological disorder. Excluding subtype cases, analysis of the effect of blood transfusion between 15 cases of same ABO genotype RBC and 5 cases of O blood type washed RBC showed no significant difference (P=0.225>0.05). Conclusions: The largest number of ABO typing discrepancy are patients with hematological disorders. Compatible ABO or same gene type RBC should be used in clinical blood transfusion. Both serological and molecular biological methods should be taken into account.

Key words: ABO blood type, Blood type identification, Transfusion

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