论著

评估新型奈瑟嗜血杆菌药敏试剂盒测试流感嗜血杆菌药敏结果

展开
  • 上海交通大学医学院附属瑞金医院微生物科,上海 200025

收稿日期: 2019-08-14

  网络出版日期: 2019-10-25

基金资助

上海市公共卫生三年行动计划重点学科建设项目(15GWZK0102)

Evaluation of TDR NH-AST kit for testing antimicrobial susceptibility of Haemophilus influenza

Expand
  • Department of Clinical Microbiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China

Received date: 2019-08-14

  Online published: 2019-10-25

摘要

目的:流感嗜血杆菌是一种可以引起严重感染的条件致病菌,故快速、准确地报告其药敏试验结果尤为重要。本研究将对某商品奈瑟菌及嗜血杆菌试剂盒(NH-AST)及相应自动分析系统检测流感嗜血杆菌的药敏结果进行评估。方法:收集101株临床标本分离得到的流感嗜血杆菌,以美国临床和实验室标准协会(Clinical and Laboratory Standards Institute,CLSI)M07-A10文件中推荐的微量肉汤稀释法作为对照方法,评估过程按照ISO20776-2标准分析NH-AST试剂盒与对照方法比较的分类一致率(category agreement,CA)、基本符合率(essential agreement,EA)、较大偏差(major discrepancy,MD)和重大偏差(very major discrepancy,VDM),并通过Kappa分析和McNemar检验分析NH-AST试剂盒与对照方法之间的一致性。结果:本研究共评估了18种抗菌药物,除美罗培南外,其余抗菌药物的药敏试验结果Kappa值均大于0.75,P值除氨苄西林外均大于0.05。CA、EA、MD和VDM总体值分别为97.80%、95.38%、2.07%、3.29%。除美罗培南、阿奇霉素和环丙沙星的EA略小于90%(分别为88.12%、84.16%、88.12%)外,其余抗菌药物全部大于90%,且全部抗菌药物CA均超过90%。结论:NH-AST商品试剂盒及其相应的检测系统简便易用,可用于流感嗜血杆菌的临床常规药敏试验。β内酰胺酶阴性但氨苄西林耐药的流感嗜血杆菌需要使用其他方法确认氨苄西林药敏结果,并慎重报告。

本文引用格式

李欣欣, 肖淑珍, 谭希婧, 倪语星, 韩立中 . 评估新型奈瑟嗜血杆菌药敏试剂盒测试流感嗜血杆菌药敏结果[J]. 诊断学理论与实践, 2019 , 18(05) : 526 -531 . DOI: 10.16150/j.1671-2870.2019.05.008

Abstract

Objective: Haemophilus influenzae is a pathogen that can cause severe infections. Accurate determination of H. influenzae susceptibility is important to ensure treatment efficiency. This study aimed to evaluatethe performance of TDR NH-AST kit for testing the antimicrobial susceptibility of Haemophilus influenzae. Methods: A total of 101 isolates were collected from clinical laboratories. Broth microdilution method was performed following the Clinical and Laboratory Standards Institute guideline M07-A10 as the reference method. The category agreement (CA), essential agreement (EA), major discrepancy (MD) and very major discrepancy (VDM) were used to evaluate the agreement between the TDR NH-AST kit and reference method according to ISO 20776-2. Kappa value was calculated and McNemar test was performed to analyze the consistency between the two methods. Results: A total of 18 antimicrobial drugs were involved. Apart from meropenem, the Kappa values of the other antimicrobial drug susceptibility test were all higher than 0.75, and the P va-lues were all higher than 0.05 except ampicillin. The overall values of CA, EA, MD and VMD were 97.80%, 95.38%, 2.07% and 3.29%, respectively. Although EAs of meropenem, azithromycin and ciprofloxacin were slightly less than 90% (88.12%, 84.16% and 88.12%, respectively), all the other antibacterial drugs were more than 90%, and CAs of all antibacterial drugs were more than 90%. Conclusions: TDR NH-AST has a good consistency, accuracy and reliability in the detection of antimicrobial susceptibility of H. influenzae, and could meet the requirements of routine clinical antimicrobial susceptibility test of H. influenzae.

参考文献

[1] Setchanova LP, Kostyanev T, Alexandrova AB, et al. Microbiological characterization of Streptococcus pneumo-niae and non-typeable Haemophilus influenzae isolates as primary causes of acute otitis media in Bulgarian children before the introduction of conjugate vaccines[J]. Ann Clin Microbiol Antimicrob, 2013, 12:6.
[2] Puig C, Grau I, Marti S, et al. Clinical and molecular epidemiology of haemophilus influenzae causing invasive disease in adult patients[J]. PLoS One, 2014, 9(11):e112711.
[3] Puig C, Calatayud L, Martí S, et al. Molecular epidemio-logy of nontypeable Haemophilus influenzae causing community-acquired pneumonia in adults[J]. PLoS One, 2013, 8(12):e82515.
[4] Levine OS, Schuchat A, Schwartz B, et al. Generic protocol for population - based surveillance of Haemophilus influenzae type B (HiB)[R/OL]. World Health Organization. 2001-05-25[2019-08-14]http://www.ripsa.org.br/lis/resource/4800.
[5] CLSI. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard-Tenth Edition[S]. Wayne,PA: Clinical and Labo-ratory Standards Institute, 2015.
[6] ISO20776-2. Clinical laboratory testing and in vitro diagnostic test systems - Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices- - -Part 2: Evaluation of performance of antimicrobial susceptibility test devices[S]. Clinical and Laboratory Standards Institute, 2007.
[7] Langereis JD, de Jonge MI. Invasive Disease Caused by Nontypeable Haemophilus influenzae[J]. Emerg Infect Dis, 2015, 21(10):1711-1718.
[8] Giufrè M, Fabiani M, Cardines R, et al. Increasing trend in invasive non-typeable Haemophilus influenzae disease and molecular characterization of the isolates, Italy, 2012-2016[J]. Vaccine, 2018, 36(45):6615-6622.
[9] Luo C, Xia Y, Liu Q, et al. Antibiotic resistance and molecular epidemiology of the beta-lactamase-producing Haemophilus influenzae isolated in Chongqing, China[J]. APMIS, 2012, 120(11):926-934.
[10] Ding Y, Geng Q, Tao Y, et al. Etiology and epidemiology of children with acute otitis media and spontaneous otorrhea in Suzhou, China[J]. Pediatr Infect Dis J, 2015, 34(5):e102-e106.
[11] Nimer NA, Al-Saa'da RJ, Abuelaish O. Accuracy of the VITEK? 2 system for a rapid and direct identification and susceptibility testing of Gramnegative rods and Gram-positive cocci in blood samples[J]. East Mediterr Health J, 2016, 22(3):193-200.
[12] Sakarikou C, Altieri A, Bossa MC, et al. Rapid and cost-effective identification and antimicrobial susceptibility testing in patients with Gram-negative bacteremia directly from blood-culture fluid[J]. J Microbiol Methods, 2018, 146:7-12.
[13] Kärpänoja P, Nissinen A, Huovinen P, et al. Disc diffusion susceptibility testing of Haemophilus influenzae by NCCLS methodology using low-strength ampicillin and co-amoxiclav discs[J]. J Antimicrob Chemother, 2004, 53(4):660-663.
[14] Idelevich EA, Becker K, Schmitz J, et al. Evaluation of an Automated System for Reading and Interpreting Disk Diffusion Antimicrobial Susceptibility Testing of Fasti-dious Bacteria[J]. PLoS One, 2016, 11(7):e0159183.
文章导航

/