目的: 比较三款常用的全自动免疫组织化学染色仪处理乳腺浸润性导管癌标本在病理诊断中的应用价值。方法: 选取30例患者的乳腺浸润性导管癌组织各1块,切取层厚3 μm的石蜡切片12张,分别使用三款常用的全自动免疫组织化学染色仪进行雌激素受体（estrogen receptor,ER）、孕激素受体（progesterone receptor,PR）、人表皮生长因子受体2（human epidermal growth factor receptor 2,HER-2）及和Ki-67染色,分析、比较采用3种仪器处理标本后,标本的4个免疫组织化学指标的阳性率。结果: 染色仪A处理的标本,ER阳性18例（10%~99%,+~+++）,PR阳性14例 （1%~99%,+~+++）,HER-2阳性29例（+~+++）,Ki-67阳性30例（5%~90%,+）;染色仪B处理的标本,ER阳性18例（10%~99%,+~+++）,PR阳性12例（1%~99%,+~+++）,HER-2阳性28例（+~+++）,Ki-67阳性30例（5%~90%,+）;染色仪C处理的标本,ER阳性18例（10%~99%,+~+++）、PR阳性12例（5%~90%,+~+++）,HER-2阳性28例（+~+++）、Ki-67阳性30例（5%~90%,+）。经卡方检验结果显示,三款免疫组织化学染色仪处理标本后,各指标间具有一致性（P>0.05）。结论: 三款常用的全自动免疫组织化学染色仪处理组织切片后, ER、PR、HER-2、Ki-67的病理诊断结果间差异无统计学意义,提示这3种仪器处理病理标本的结果具有一致性。
Objective: To investigate the performance of 3 types of automatic immunohistochemical stainer(Leica Bond-Max, Dako Omnis and BenchMark Ultra automatic immunohistochemical stainer) in pathological diagnosis of invasive ductal carcinoma of breast. Methods: Paraffin-embedded tissue blocks from 30 patients with infiltrating ductal carcinoma were collected,and 12 sections with a thickness of 3 μm were excised from each paraffin-embedded tissue block. After being stained with these three types of automatic immunohistochemical stainer, the specimens were examined to determine the expressions of estrogen receptor(ER）, progesterone receptor (PR）, human epidermal growth factor receptor 2（HER-2） and Ki67. Positive rates of above mentioned parameters between specimens prepared with these three different stainers were compared. Results: For Leica Bond-Max, the cases with positive ER(10%-99%, +~+++）, PR(1%-99%, +~+++）, HER-2（+~+++） and Ki-67（5%-90%, +）were 18, 14, 29 and 30 cases, respectively, by Dako Omnis were 18, 12, 28 and 30 cases, respectively, and by BenchMark Ultra were 18, 12, 28 and, 30 cases, respectively. There were no significant differences in positive expressions of these parameters in specimens prepared with these 3 types of automatic immunohistochemical stainer(P>0.05). Conclusions: Pathologic results of ER, PR, HER-2 and Ki-67 expression in breast carcinoma tissue were similar when specimens were prepared with these three types of automatic immunohistochemical stainer, indicating that specimens could be prepared with any type of above mentioned automatic immunohistochemical stainer.
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