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微阵列酶联免疫荧光法和免疫印迹法检测过敏原特异性IgE的结果分析

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  • 上海中医药大学附属龙华医院检验科,上海 200032

收稿日期: 2017-03-20

  网络出版日期: 2017-06-25

Analysis of performance of microfluid chip ELFA and IBT for detecting allergen specific IgE

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  • Department of Clinical Laboratory, Longhua Hospital, Shanghai 200032, China

Received date: 2017-03-20

  Online published: 2017-06-25

摘要

目的: 比较微阵列酶联免疫荧光法(enzyme-linked fluoroimmunoassay,ELFA)与免疫印迹法(immunoblotting test,IBT)2种过敏原筛查方法。方法: 利用多种IgE抗体强阳性混合人类血清和马血血清,对微阵列ELFA(以下简称ELFA)与IBT 这2种过敏原筛查方法的稳定性、稀释能力进行比对,并取其共有的过敏原进行阳性分级一致性比对。结果: ELFA检测尘螨、蟹、虾、花生、鸡蛋白、狗毛屑等6项过敏原的变异系数(coefficient of variation, CV%)均值明显低于IBT(T=6.20,P<0.01),而对于猫毛屑、蟑螂、牛奶等3项过敏原,ELFA的CV%均值略高于IBT,但差异尚无统计学意义(T=2.43,P>0.05)。对于不同稀释度的样本,2种方法的检测结果均与其稀释浓度呈正相关。2种方法间对屋尘螨及蟑螂的阳性分级一致性程度较高,而对花生、鸡蛋白、狗毛屑、猫毛屑、牛奶等5项过敏原的阳性分级一致性较差。结论: ELFA与IBT在过敏原检测中存在一定差异,但2种方法的检测结果均可很好地显示过敏患者血清中的特应性IgE浓度。与IBT相比,ELFA具有高稳定性、检测项目多、检测时间短、标本用量少等特点。

本文引用格式

赵婕, 赵凌旭, 胡晓波 . 微阵列酶联免疫荧光法和免疫印迹法检测过敏原特异性IgE的结果分析[J]. 诊断学理论与实践, 2017 , 16(03) : 306 -312 . DOI: 10.16150/j.1671-2870.2017.03.015

Abstract

Objective: To compare the performance of BioIC Allergen specific IgE Detection Kit (micro-fluid chip enzyme-linked fluoroimmunoassay, ELFA) and EUROLINE Atopy China (immunoblotting test, IBT) for detecting allergen-specific IgE. Methods: The stability and dilution of these two methods were compared by using mixed human plasma with strong positivity for multi-sIgE and horse plasma, and the positive levels of common allergens were also compared. Results: The CV% of ELFA was obviously lower than that of IBT in six kinds of allergens including mite mix, crab, shrimp, peanut, egg white and dog dander(T=6.20, P<0.01), while was slight higher in cat dander,cockroach and milk(T=2.43, P>0.05). Both IBT and ELFA showed a positive correlation of testing result with concentration dilution. There was a high consistency of positive grading for mite mix and cockroach, while a weak consistency for peanuts and other five kinds of allergens. Conclusions: There are some differences between ELFA and IBT, but both could reflect the concentration of sIgE. Compared with IBT, ELFA has a higher stability and sensitivity, covering more allergens, less testing time and sample volume. ELFA could be used in allergen-specific IgE testing in clinical laboratory.

参考文献

[1] 邹寒冰, 徐以平. 32例过敏疾病患者血清14种食物过敏原特异性IgG抗体的检测[J]. 标记免疫分析与临床,2006,13(1):13-15.
[2] 谈丰平, 朱翠玉. 32例过敏性哮喘患者血清中总IgE和四种过敏原特异性IgE的检测[J]. 标记免疫分析与临床,1994,1(4):229-230.
[3] Kamble S, Bharmal M.Incremental direct expenditure of treating asthma in the United States[J]. J Asthma,2009, 46(1):73-80.
[4] 罗茂红, 来则民. 家庭环境因素与儿童哮喘关系的病例对照研究[J]. 中国公共卫生,2002,18(6):662-663.
[5] 张春梅, 陈蕴光, 赖荷. 14种食物过敏原特异性IgG抗体与变态反应性皮肤病的关系分析[J]. 表及免疫分析与临床,2010,17(1):12-15.
[6] 杨德平. 变态反应性皮肤病患者过敏原体外检测结果分析[J]. 检验医学与临床,2015,12(10):1437-1438.
[7] 曲志春, 丁晓燕, 赵云学, 等. 变态反应性疾病患者血清总IgE及特异性IgE检测结果分析[J]. 中国临床研究,2010,23(5):405-406.
[8] 杨德平. 450例患者过敏原体外检测结果分析[J]. 国际检验医学杂志,2015,36(10):1453-1455.
[9] 杨德平. 过敏性疾病患者过敏原检测结果分析[J]. 国际检验医学杂志,2015,36(7):987-988.
[10] 汤少珊, 梁少媛, 陈广道. 广州地区过敏性疾病儿童血清特异性过敏原IgE检测分析[J]. 广州医药,2015,46(4):63-65.
[11] 胡斌, 李平立, 文东菁, 等. 过敏性皮肤病血清总IgE和过敏原特异性IgE检测分析[J]. 医学综述,2015,21(8):1531-1532.
[12] 孙振燕, 章婧, 郑建峰, 等. 急性荨麻疹患者133例临床分析[J]. 当代临床医刊,2015,28(3):1374-1375.
[13] 李晓琼, 杨春华, 潘邵武, 等. 面向POCT应用的微流控芯片技术综述[J]. 世界复合医学,2015,3(1):30-37.
[14] 邵莉, 曹兰芳, 李晓丽, 等. 支气管哮喘合并肺炎支原体感染小鼠模型白介素9、白介素17、γ干扰素的变化[J]. 内科理论与实践,2016,11(1):41-51.
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