目的: 比较微阵列酶联免疫荧光法(enzyme-linked fluoroimmunoassay,ELFA)与免疫印迹法(immunoblotting test,IBT)2种过敏原筛查方法。方法: 利用多种IgE抗体强阳性混合人类血清和马血血清,对微阵列ELFA(以下简称ELFA)与IBT 这2种过敏原筛查方法的稳定性、稀释能力进行比对,并取其共有的过敏原进行阳性分级一致性比对。结果: ELFA检测尘螨、蟹、虾、花生、鸡蛋白、狗毛屑等6项过敏原的变异系数(coefficient of variation, CV%)均值明显低于IBT(T=6.20,P<0.01),而对于猫毛屑、蟑螂、牛奶等3项过敏原,ELFA的CV%均值略高于IBT,但差异尚无统计学意义(T=2.43,P>0.05)。对于不同稀释度的样本,2种方法的检测结果均与其稀释浓度呈正相关。2种方法间对屋尘螨及蟑螂的阳性分级一致性程度较高,而对花生、鸡蛋白、狗毛屑、猫毛屑、牛奶等5项过敏原的阳性分级一致性较差。结论: ELFA与IBT在过敏原检测中存在一定差异,但2种方法的检测结果均可很好地显示过敏患者血清中的特应性IgE浓度。与IBT相比,ELFA具有高稳定性、检测项目多、检测时间短、标本用量少等特点。

Objective: To compare the performance of BioIC Allergen specific IgE Detection Kit (micro-fluid chip enzyme-linked fluoroimmunoassay, ELFA) and EUROLINE Atopy China (immunoblotting test, IBT) for detecting allergen-specific IgE. Methods: The stability and dilution of these two methods were compared by using mixed human plasma with strong positivity for multi-sIgE and horse plasma, and the positive levels of common allergens were also compared. Results: The CV% of ELFA was obviously lower than that of IBT in six kinds of allergens including mite mix, crab, shrimp, peanut, egg white and dog dander(T=6.20, P<0.01), while was slight higher in cat dander,cockroach and milk(T=2.43, P>0.05). Both IBT and ELFA showed a positive correlation of testing result with concentration dilution. There was a high consistency of positive grading for mite mix and cockroach, while a weak consistency for peanuts and other five kinds of allergens. Conclusions: There are some differences between ELFA and IBT, but both could reflect the concentration of sIgE. Compared with IBT, ELFA has a higher stability and sensitivity, covering more allergens, less testing time and sample volume. ELFA could be used in allergen-specific IgE testing in clinical laboratory.