收稿日期: 2019-11-21
网络出版日期: 2022-07-14
基金资助
国家自然科学基金(81971509);国家自然科学基金(8187126);上海申康医院发展中心(16CR3098B);上海市“医苑新星”青年医学人才培养资助计划-临床检验项目
Epidemiological analysis of human papilloma virus infection in male patients with condyloma acuminatum during 2017-2018 in Shanghai
Received date: 2019-11-21
Online published: 2022-07-14
目的: 分析上海地区男性尖锐湿疣患者的人乳头瘤病毒(human papilloma virus,HPV)感染流行病学特征,为该病的防治提供依据。方法: 选取2017年3月至2018年3月期间,本院皮肤科门诊确诊的男性尖锐湿疣患者2 202例,采用PCR-反向点杂交法检测皮损部位的HPV感染情况及基因亚型。结果: 2 202例男性尖锐湿疣患者,HPV DNA阳性者有1 136例,阳性率达51.59%。HPV基因型别分布中,低危型以6型和11型阳性率较高,分别为42.78%(486/1 136)和27.02%(307/1 136);高危型以16型、52型和51型阳性率较高,分别为12.06%(137/1 136)、10.83%(123/1 136)和7.22%(82/1 136)。分析感染模式发现,1 136例HPV DNA阳性患者中,以单一感染为主,占所有阳性患者的56.78%(645/1 136);多重感染模式中,以二重感染为主,占所有阳性患者的25.62%(291/1 136)。单纯低危型HPV感染患者占44.45%(505/1 136),最高可达三重感染;其次为高低危混合型感染,占34.07%(387/1 136),最高可达十重感染;单纯高危型HPV感染占21.48%(244/1 136),可达六重感染。年龄41~60岁组内阳性检出率最高(60.35%,207/343),与≤20岁组(49.00%,49/100)及21~40岁组(49.43%,819/1 657)比较, χ2值分别为4.09和13.57,P值均<0.05。比较各年龄组的感染模式发现,21~40岁组以单一基因亚型感染为主(59.71%,489/819);≤20岁组及≥61岁组以多重感染为主,百分比分别为55.10%(27/49)和59.02%(36/61)。结论: 上海地区男性尖锐湿疣患者的HPV DNA阳性率为51.59%,较河南、浙江及广东东莞地区低;感染亚型主要为6型、11型、16型、52型和51型,感染模式以单一亚型感染和低危型感染为主,41~60岁年龄组为高发人群,而≤20岁和≥61岁以上人群的多重感染百分比明显升高。
王娟, 李佳 . 2017年至2018年上海地区男性尖锐湿疣患者人乳头瘤病毒感染的流行病学分析[J]. 诊断学理论与实践, 2020 , 19(06) : 572 -576 . DOI: 10.16150/j.1671-2870.2020.06.005
Objective: To investigate the epidemiological characteristics of human papilloma virus (HPV) infection in male patients with condyloma acuminatum in Shanghai, and to provide evidence for prevention and treatment of the disease. Methods: A total of 2 202 male patients with condyloma acuminatum diagnosed in the dermatology clinic of our hospital were enrolled between March 2017 and March 2018, and HPV infection status was explored. The HPV genotypes in skin lesion were tested with polymerase chain reaction (PCR)-reverse dot blot hybridization. Results: It showed 1 136 out of 2 202 patients were HPV DNA positive and positive rate was 51.59%. The positive rates of low-risk HPV 6 and 11 were 42.78% and 27.02%, while the high-risk HPV 16, 52 and 51 were 12.06%, 10.83% and 7.22% respectively. In 1 136 cases with positive HPV DNA, single infection was the main pattern with a positive rate of 56.78%. In patients with multiple HPV infection, double infection was the main form, accounting for 25.62%. It showed that 44.45% patients were infected with low-risk genotypes of HPV (single,double or triple HPV genotype infection), 34.07% patients had mixed infection of high-risk and low risk genotypes ( up to ten infections); 21.48% were only high-risk HPV infection (up to six infections). The positive detection rate in 41-60 year group was the highest (60.35%), which was significantly higher than the groups of ≤20 year and 21-40 year (all P<0.05, χ2=4.09 and χ2=13.57). Infection patterns among different age groups were as follows: 21-40 year patients were mainly infected by one single genotype,and majority patients in both≤20 year and ≥61 year group had multiple HPV infection. Conclusions: The detection rate of positive HPV DNA in male patient with condyloma acuminatum in Shanghai is 51.59%, and lower than the rates reported in Henan, Zhejiang and Dongguan(Guangdong), and the genotypes include HPV 6,11,16,52,51.The infection was mainly caused by single and low-risk HPV. Detection rate in 41-60 year male is the highest, and the proportion of multiple infections are higher in people ≤20 years and ≥61 years.
Key words: Male; Condyloma acuminatum; Human papilloma virus; Genotype; Epidemiology
[1] | Zhao X, Wu Q, Wang X, et al. The performance of human papillomavirus DNA detection with type 16/18 genotyping by hybrid capture in primary test of cervical cancer screening: a cross-sectional study in 10,669 Chinese women[J]. Clin Microbiol Infect, 2018, 24(12):1322-1327. |
[2] | Moscicki AB, Palefsky JM. Human papillomavirus in men: an update[J]. J Low Genit Tract Dis, 2011, 15(3):231-234. |
[3] | Nowińska K, Ciesielska U, Podhorska-Okołów M, et al. The role of human papillomavirus in oncogenic transformation and its contribution to the etiology of precance-rous lesions and cancer of the larynx: a review[J]. Adv Clin Exp Med, 2017, 26(3):539-547. |
[4] | Han JJ, Beltran TH, Song JW, et al. Prevalence of genital human papillomavirus infection and human papillomavirus vaccination rates among US adult men: National Health and Nutrition Examination Survey (NHANES) 2013-2014[J]. JAMA Oncol, 2017, 3(6):810-816. |
[5] | Giuliano AR, Palefsky JM, Goldstone S, et al. Efficacy of quadrivalent HPV vaccine against HPV Infection and disease in males[J]. N Engl J Med, 2011, 364(5):401-411. |
[6] | Deshmukh AA, Tanner RJ, Luetke MC, et al. Prevalence and risk of penile human papillomavirus infection: evidence from the National Health and Nutrition Examination Survey 2013-2014[J]. Clin Infect Dis, 2017, 64(10):1360-1366. |
[7] | Bzhalava D, Eklund C, Dillner J. International standardi-zation and classification of human papillomavirus types[J]. Virology, 2015, 476:341-344. |
[8] | Nogueira Dias Genta ML, Martins TR, Mendoza Lopez RV, et al. Multiple HPV genotype infection impact on invasive cervical cancer presentation and survival[J]. PLoS One, 2017, 12(8):e0182854. |
[9] | Hajia M, Sohrabi A. Possible synergistic interactions among multiple HPV genotypes in women suffering from genital neoplasia[J]. Asian Pac J Cancer Prev, 2018, 19(3):785-789. |
[10] | Yang Y, Jia CW, Ma YM, et al. Correlation between HPV sperm infection and male infertility[J]. Asian J Androl, 2013, 15(4):529-532. |
[11] | Meites E, Kempe A, Markowitz LE. Use of a 2-dose schedule for human papillomavirus vaccination - updated recommendations of the advisory committee on immunization practices[J]. MMWR Morb Mortal Wkly Rep, 2016, 65(49):1405-1408. |
[12] | Harrison C, Britt H, Garland S, et al. Decreased management of genital warts in young women in Australian gene-ral practice post introduction of national HPV vaccination program: results from a nationally representative cross-sectional general practice study[J]. PLoS One, 2014, 9(9):e105967. |
[13] | Ali H, Guy RJ, Wand H, et al. Decline in in-patient treatments of genital warts among young Australians following the national HPV vaccination program[J]. BMC Infect Dis, 2013, 13:140. |
[14] | 冯莉亚, 刘军权, 陈莹莹. 反向点杂交法测定浙江省4 620例男性HPV感染者的流行病学特征[J]. 中华临床实验室管理电子杂志, 2019, 7(1):26-30. |
[15] | 钟阳青, 陈艳清, 肖翔, 等. 东莞地区男性生殖道HPV感染率及基因型分布特点分析[J]. 实验与检验医学, 2018, 36(5):781-783. |
[16] | 陈光辉, 赵静, 艾予蜀, 等. 男性尖锐湿疣患者人乳头瘤病毒感染状况分析[J]. 中华传染病杂志, 2018, 36(5):277-279. |
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