目的 采用高通量转录组测序技术,通过生物信息学手段,初步筛选并鉴定结肠直肠癌病人术前新辅助治疗效果相关基因,并进行差异表达分析,为进一步阐明结肠直肠癌新辅助治疗敏感性的分子机制提供基础。方法 22例接受标准术前新辅助治疗的结肠直肠癌病人,根据肿瘤消退评级分为敏感组9例,不敏感组13例。提取肿瘤组织RNA,构建转录组文库,采用高通量测序平台进行测序。结合生物信息学等相关手段,筛除错误发生率（<0.05）的基因后对比组装,对得到的转录组样本进行分类和注释。对差异表达基因特征进行分析。结果 初步建立结肠直肠癌新辅助治疗敏感性基因数据库。从22例转录组标本中,发现89个基因表达出现显著上调,112个基因出现显著下调。结论 高通量测序分析提示,这些基因表达变化可能与结肠直肠癌新辅助治疗敏感性密切相关。
Objective To screen and identify the genes associated with sensitivity of neoadjuvant therapy for the patients with colorectal cancer through high-throughput transcriptome sequence technology and bioinformatics tools by analysis of the genes which expressed differently. Methods RNA was extracted from the tumor tissue of 22 patients with co-lorectal cancer who received standard neoadjuvant therapy before operation. Nine cases were divided as sensitive group and 11 cases as non-sensitive group based on tumor regression grading. Strand libraries were constructed for high-throughput sequencing. The genes filtered the false discovery rate(<0.05) were mapped to the reference genome, and assembled. The obtained transcripts were classified and annotated and the characteristics of gene were analyzed. Results The gene database associated with neoadjuvant therapy for colorectal cancer was built preliminarily. From 22 patients with colorectal cancer tissues, 89 genes were found up-regulated significantly and 112 genes down-regulated. Conclusions The genes related to the sensitivity of colorectal cancer neoadjuvant therapy were identified in this study using high-throughput transcriptome sequence.
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