目的： 分离和鉴定甲状腺乳头状癌KTC-1细胞分泌的外泌体,并探讨外泌体携带miRNA146b-5p对KTC-1细胞体外迁移和侵袭的影响。方法： 收集KTC-1细胞的外泌体,电转miRNA146b-5p和其抑制剂进入外泌体,并与KTC-1细胞共培养。检测KTC-1细胞的体外迁移和侵袭的能力。结果： 成功分离和鉴定KTC-1细胞分泌的外泌体。外泌体miRNA146b-5p可进入KTC-1细胞,促进KTC-1细胞的体外迁移和侵袭。结论： 外泌体miRNA146b-5p可促进甲状腺乳头状癌的体外侵袭和转移。外泌体miRNA423-5p沉默可能作为甲状腺乳头状癌的治疗方法。
Objective To isolate and identify the exosomes secreted by papillary thyroid carcinoma (PTC) KTC-1 cells, and to investigate the effects of miRNA146b-5p in exosomes on the migration and invasion in vitro. Methods Exosomes secreted by KTC-1 cells were collected and identified. miRNA 146b-5p and its inhibitor were electroporated into exosomes and co-cultured with KTC-1 cells. Migration and invasion in vitro were examined. Results Exosomes secreted by KTC-1 cells were successfully isolated and identified. Exosomal miRNA146b-5p could enter KTC-1 cells and promote migration and invasion of KTC-1 cells in vitro. Conclusions Exosomal miRNA146b-5p can promote the migration and invasion of PTC in vitro. miRNA146b-5p silencing in exosomes might represent a potential approach against PTC.
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