1.Key Laboratory of Urban Agriculture(South), Ministry of Agriculture, School of Agriculture and Biology, Shanghai Jiaotong University; Shanghai 200240, China 2.School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney 2052, Australia
Through the simulation test, PCR-DGGE method was used to investigate the effects of different carbon and nitrogen organic materials on bacterial community structure of organic vegetable production system soil.Results showed that bacterial community structure of conventional and organic production system soils were significantly different.By analyzing the Shannon-Wiener diversity index, overall the bacterial diversity of organic background soil treatments was higher than conventional background soil treatments.The bacterial diversity of organic production system soil with straw(OS)was the highest.Bacterial diversity decrease or increase after adding urea or alfalfa respectively.Conventional and organic production system soils were divided into two ethnic clusters by clustering analysis.Phylogenetic tree showed that the 30 closely related species were attributed to Proteobacteria, Acidobacteria, Actinobacteria, Firmicutes, Verrucomicrobia.The band B13 of CA(conventional production system soil with alfalfa)and the common band B28 of organic background treatment had the highest homology with Bacillus and Pseudomonas respectively.
Uncultured bacterium isolate DGGE gel band CB-16 16S ribosomal RNA gene, partial sequence(KJ667555)
94%
Tab.3 细菌DGGE图谱条带的序列比对结果
Fig.3 细菌的系统发育树
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