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2016 , Vol. 34 >Issue 1: 11 - 15

DOI: https://doi.org/10.3969/J.ISSN.1671-9964.2016.01.003

牛磺酸对昆明小鼠早期胚胎体外发育和着床的影响

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  • 1.潍坊学院 生物与农业工程学院, 山东 潍坊 261061
    2.山东省高校生物化学与分子生物学重点实验室(潍坊学院), 山东 潍坊 261061
    3.潍坊学院化学化工与环境工程学院, 山东 潍坊261061

作者简介: 范晶晶(1981-), 女, 博士, 讲师, 研究方向:动物生殖与发育研究, E-mail:fanjingjing2008@163.com

收稿日期: 2015-02-01

  网络出版日期: 2016-03-08

基金资助

山东省自然科学基金项目(ZR2013BL015);潍坊市科学技术发展计划(20121307);潍坊学院博士基金项目(2010BS04)

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Effectof Taurine on Development and Implantation of Kunming Mouse Early Embryos

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  • 1. Biological and Agricultural Engineering College ofWeifang University, Weifang 261061, China
    2. Key Laboratory of Biochemistry & Molecular Biology in Universities of Shandong, Weifang University, Weifang 261061 China
    3. College of Chemistry and Chemical Engineering, Weifang University, Weifang 261061, China

Received date: 2015-02-01

  Online published: 2016-03-08

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摘要

本研究主要探讨了牛磺酸对昆明(KM)小鼠体外胚胎发育及对胚胎着床的作用。在mKSOM(modified potassium simplex optimized medium)胚胎体外培养液中分别添加不同浓度牛磺酸, 培养24、72 h后分别统计4-细胞率、囊胚率、囊胚总细胞数; 采用子宫角注射的方法, 研究牛磺酸转运抑制剂β-丙氨酸对孕D9胚胎着床数的影响。结果表明, 添加10 mmol/L牛磺酸培养液组的胚胎4-细胞率高于对照组(P<0.05), 囊胚率与囊胚总细胞数明显高于对照组(P<0.01)。子宫角注射β-丙氨酸(20 μg/mL)显著降低D9着床的胚胎数。本研究初步明确了牛磺酸对小鼠体外胚胎发育及对胚胎着床的影响, 为进一步研究胚胎发育和着床提供参考。

关键词： 牛磺酸; 胚胎发育; 着床

本文引用格式

范晶晶, 郑秋闿 . 牛磺酸对昆明小鼠早期胚胎体外发育和着床的影响[J]. 上海交通大学学报(农业科学版), 2016 , 34(1) : 11 -15 . DOI: 10.3969/J.ISSN.1671-9964.2016.01.003

Abstract

The effects of taurine on development and implantation of Kunming mouse early embryos were investigated.The embryos were cultivated in modified potassium simplex optimized medium(mKSOM)with different concentration of taurine.The percentage of 4-cell, blastocysts and average total cell number of blastocysts were calculated after 24 h and 72 h.Cornua uteri injection technique was used to investigate the effects of β-alaline(β-ala, taurine transport inhibitor)on embryo implantation.The results showed that there were significant differences between 10 mmol/L taurine treatment and control in the percentage of 4-cell(P<0.05), blastocysts and total cell number of blastocysts(P<0.01).Meanwhile, injection of β-ala(20 μg/mL)into uterine horn significantly reduced the number of implanted embryos on D9.This study explicated the effects of taurine on the development and implantation of early Kunming mouse embryos, providing a useful reference for further uncovering of mechanism of embryo development and implantation.

Key words： taurine; embryo development; implantation

参考文献

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