不同碳氮有机物料对有机菜田土壤细菌多样性的影响

杨昊, 张瑞, 叶俊, 蔡保松, 黄丹枫

doi:10.3969/J.ISSN.1671-9964.2016.01.007

上海交通大学学报(农业科学版) >

2016 , Vol. 34 >Issue 1: 36 - 44

DOI: https://doi.org/10.3969/J.ISSN.1671-9964.2016.01.007

不同碳氮有机物料对有机菜田土壤细菌多样性的影响

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1.上海交通大学农业与生物学院, 农业部都市农业(南方)重点实验室, 上海200240
2.新南威尔士大学生物技术学院, 悉尼2052

作者简介: 杨昊(1988-), 男, 硕士生, 研究方向:菜田土壤微生物多样性, E-mail: yanghaoiloveu@163.com;

通讯作者: 黄丹枫(1956-), 女, 教授, 博士生导师, 研究方向:园艺作物生理生态, E-mail:hdf@sjtu.edu.cn; 蔡保松(1971-), 男, 副教授, 硕士生导师, 研究方向:园艺作物生理生态, E-mail:czstl@sjtu.edu.cn

收稿日期: 2014-12-22

网络出版日期: 2016-03-08

基金资助

上海市科委(12231205103);上海市农委[沪农科攻字(2012)第 2 号];SJTU-UNSW联合研究与发展基金(13X120020003);上海市园艺学重点学科培育与建设项目资助

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Effect of Bacterial Community Structure Diversity with Different Carbon and Nitrogen Organic Materials Treatments in Organic Vegetable Soil

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1.Key Laboratory of Urban Agriculture(South), Ministry of Agriculture, School of Agriculture and Biology, Shanghai Jiaotong University; Shanghai 200240, China
2.School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney 2052, Australia

Received date: 2014-12-22

Online published: 2016-03-08

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摘要

通过模拟试验, 利用聚合酶链式反应-变性梯度凝胶电泳法(PCR-DGGE), 研究了施入不同碳氮有机物料(秸秆、苜蓿、有机肥、尿素)56 d后, 有机生产系统菜田土壤细菌群落结构的特征。结果表明, 常规和有机生产系统土壤的细菌群落结构有明显差异。由DGGE图谱Shannon-Wiener多样性指数分析得知, 有机背景处理的细菌多样性整体高于常规背景处理, 且有机生产系统土壤加秸秆处理(OS)多样性最高, 加入尿素后细菌多样性降低, 相反, 加入苜蓿后细菌多样性升高。非加权组平均法(UPGMA)聚类分析将常规和有机背景土壤分为两大族群。DGGE条带测序和系统进化树表明, 30个条带归属为Proteobacteria、Acidobacteria、Actinobacteria、Firmicutes、Verrucomicrobia。常规土壤加苜蓿(CA)处理出现的特征性条带B13与有机背景处理的共有条带B28分别与Bacillus属和Pseudomonas假单胞菌属同源性最高。

关键词： 秸秆; 苜蓿; 尿素; 有机土壤; PCR-DGGE; 细菌群落结构

本文引用格式

杨昊, 张瑞, 叶俊, 蔡保松, 黄丹枫 . 不同碳氮有机物料对有机菜田土壤细菌多样性的影响[J]. 上海交通大学学报(农业科学版), 2016 , 34(1) : 36 -44 . DOI: 10.3969/J.ISSN.1671-9964.2016.01.007

Abstract

Through the simulation test, PCR-DGGE method was used to investigate the effects of different carbon and nitrogen organic materials on bacterial community structure of organic vegetable production system soil.Results showed that bacterial community structure of conventional and organic production system soils were significantly different.By analyzing the Shannon-Wiener diversity index, overall the bacterial diversity of organic background soil treatments was higher than conventional background soil treatments.The bacterial diversity of organic production system soil with straw(OS)was the highest.Bacterial diversity decrease or increase after adding urea or alfalfa respectively.Conventional and organic production system soils were divided into two ethnic clusters by clustering analysis.Phylogenetic tree showed that the 30 closely related species were attributed to Proteobacteria, Acidobacteria, Actinobacteria, Firmicutes, Verrucomicrobia.The band B13 of CA(conventional production system soil with alfalfa)and the common band B28 of organic background treatment had the highest homology with Bacillus and Pseudomonas respectively.

Key words： straw; alfalfa; urea; organic soil; PCR-DGGE; bacterial community structure

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