组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2010, Vol. 6 ›› Issue (5): 248-252.doi: 10.3969/j.issn.1673-0364.2010.05.003

• 论著 • 上一篇    下一篇

IFN-γ上调MHC-Ⅱ分子表达对KC免疫原性的影响

宋仁刚，宋任强，柳大烈   

  1. 广东省深圳市深圳市人民医院；黑龙江省佳木斯市佳木斯市疾病控制中心；广东省广州市南方医科大学珠江医院整形科
  • 发布日期:2020-07-23

The Effect of IFN-γ Increasing MHC-Ⅱ Expressing to the Immunogenicity of Keratinocytes

SONG Rengang1,SONG Renqiang2,LIU Dalie3   

  1. 1 Department of Plastic Surgery,Shenzhen People′s Hospital,Shenzhen 518020,China;2 Jiamusi Municipal Center for Disease Control and Prevention,Jiamusi 154002,China;3 Department of Plastic Surgery,Zhujiang Hospital,Southern Medical University,Guangzhou 510282,China.
  • Published:2020-07-23
  • Contact: 国家自然科学基金(30570517)

摘要： 目的观察在炎症因子IFN-γ的作用下,角质形成细胞（Keratinocyte,KC）免疫原性的变化情况。方法 KC体外传代培养,按不同的INF-γ浓度分组后作用于传代的KC。将高表达MHC-Ⅱ分子的KC与异体淋巴细胞混合培养后,观察淋巴细胞的增殖情况;同时,将KC植入异体小鼠皮下,观察免疫排斥迹象。结果在6 000 U/mL IFN-γ的作用下,MHC-Ⅱ+的KC超过90%。高表达MHC-Ⅱ分子的传代KC无明显刺激异体淋巴细胞增殖的作用,植入异体小鼠皮下后也无明显淋巴细胞浸润现象。结论传代后的KC在IFN-γ作用下高表达MHC-Ⅱ分子,表达MHC-Ⅱ分子的KC无明显免疫原性。

关键词: 干扰素-γ, 主要组织相容性复合体-Ⅱ, 角质形成细胞, 免疫原性

Abstract: Objective To observe changes of immunogenicity of keratinocytes（KC） under the influence of interferon-γ（IFN-γ）.Methods KC were cultured and passaged in vitro.Different dose of IFN-γ stimulated KC to express MHC-Ⅱ molecules.KC with highty expressed MHC-Ⅱ molecules were mixed with allogenic lymphocytes to observe their proliferation.Simultaneously,MHC-Ⅱ+ KC were implanted subcutaneously into allogenic mice to observe the sign of immunological rejection.Results Under the influence of 6 000 U/mL IFN-γ,the percentage of MHC-Ⅱ+ KC exceeded 90%.MHC-Ⅱ molecules highly expressed KC had no effects to stimulate proliferation of allogenic lymphocytes significantly.There were no obvious lymphocytes infiltration when MHC-Ⅱ molecules highly expressed KC were implanted subcutaneously into allogenic mice.Conclusion Passaged KC could express MHC-Ⅱ molecules highty in the influence of IFN-γ,KC expressed MHC-Ⅱ molecules has no obvious immunogenicity.

Key words: IFN-γ, MHC-Ⅱ, Keratinocyte, Immunogenicity

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