组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2012, Vol. 8 ›› Issue (1): 6-31.doi: 10.3969/j.issn.1673-0364.2012.01.002

• 论著 • 上一篇    下一篇

酶洗法制备兔组织工程气管支架的周期研究

李建忠，张志培，汪健，吴凡，金岩，韩勇，姜涛，闫小龙，倪云峰，赵晋波，李小飞   

  1. 第四军医大学唐都医院胸腔外科
  • 发布日期:2020-07-23

The Optimal Cycle of Detergent-enzymatic Method for Rabbit Tissue Engineering Tracheal Matrix

LI Jianzhong,ZHANG Zhipei,WANG Jian,WU Fan,JIN Yan,HAN Yong,JIANG Tao,YAN Xiaolong,NI Yunfeng,ZHAO Jinbo,LI Xiaofei   

  1. Department of Thoracic Surgery,Tangdu Hospital,The Fourth Military Medical University,Xi’an 710038,China.
  • Published:2020-07-23

摘要： 目的探讨酶洗法制备兔气管支架的最适宜处理周期。方法健康新西兰兔24只,随机分为4组(n=6)。以新鲜未处理气管作为对照组(A组),按照酶洗法2、3、4个周期处理气管作为实验组(B组、C组、D组)。对照组和实验组标本处理后光镜组织学观察,电镜扫描观察超微结构,并测量气管最大拉伸力、破裂力、变异率等生物力学性能。结果B、C组气管支架的最大拉伸力、最大破裂力和变异率均显著大于A、D组;组织学观察显示A组有大量黏膜上皮细胞,B组见部分黏膜上皮细胞,C、D组未见黏膜上皮细胞;电镜扫描显示实验A、B、C组气管支架的细胞基质和胶原纤维明显多于D组。结论经酶洗法处理3个周期的兔气管支架具有理想的去抗原效果和生物力学性能,并具有较完整的细胞外基质,适合用于构建组织工程同种异体气管。

关键词: 组织工程, 酶洗法, 生物力学, 细胞外基质

Abstract: Objective To explore the optimal cycle of detergent-enzymatic method for rabbit tissue engineering tracheal matrix.Methods Twenty-four healthy New Zealand rabbits were randomly divided into 4 groups(n=6),the tracheas of group A was treated with nothing as control group,the tracheas of group B,C and D were treated with two,three and four cycles of the detergent-enzymatic method separately.The histologic appearance,ultrastructure,maximum drawing force,rupture force and mutation rate were observed.Results The maximum drawing force,rupture force and mutation rate on group B and group C were bigger than group A and group D.There were many epithelial cells in the tracheas of group A,some epithelial cells in the tracheas of group B,but none epithelial cells in the tracheas of group C and group D by histological observation.The extracellular matrix and collagen fiber in group A,B,C were significantly more than in group D by SEM.Conclusion The tracheals treated with three cycles are more nonimmunogenic,have better biomechanical performance and more ECM.It is suitable for reconstruction of allograft engineering tracheal.

Key words: Tissue engineering, Detergent-enzymatic method, Biomechanics, Extra cellular matrix(ECM)

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