组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2012, Vol. 8 ›› Issue (2): 73-77.doi: 10.3969/j.issn.1673-0364.2012.02.004

• 论著 • 上一篇    下一篇

丹参酮ⅡA诱导瘢痕疙瘩成纤维细胞凋亡及细胞周期阻滞的实验研究

陈刚，梁奕敏，李青峰   

  1. 南方医科大学附属珠江医院整形外科；上海交通大学医学院附属第九人民医院整复外科
  • 发布日期:2020-07-23

Effect of Tanshinone ⅡA on Cell Proliferation,Apoptosis and Cell Cycle of Fibroblasts Derived from Keloid

CHEN Gang1,LIANG Yimin2,LI Qingfeng2   

  1. 1 Department of Plastic Surgery,Zhujiang Hospital,Southern Medical University,Guangzhou 510280,China;2 Department of Plastic and Reconstructive Surgery,Shanghai Ninth People’s Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 20011,China.
  • Published:2020-07-23

摘要： 目的观察丹参酮ⅡA对人瘢痕疙瘩成纤维细胞增殖、凋亡及细胞周期的影响。方法体外培养人瘢痕疙瘩成纤维细胞,予含有不同浓度丹参酮ⅡA的培养液(分别为0μg/mL、50μg/mL、100μg/mL和200μg/mL)进行干预。不同时间点以CCK-8检测细胞增殖活力,流式细胞仪检测细胞凋亡、细胞周期的变化。结果不同浓度丹参酮ⅡA干预和不同时间干预,对瘢痕疙瘩成纤维细胞的增殖活性、早期凋亡率、细胞周期G0/G1期比例的影响,均具有统计学意义(P<0.001),其中200μg/mL组干预效果最明显。结论丹参酮ⅡA具有抑制瘢痕疙瘩成纤维细胞增殖,诱导凋亡并阻滞细胞周期的作用,且干预效果存在浓度依耐性及时间依耐性。

关键词: 瘫痕疙瘩, 成纤维细胞, 丹参酮ⅡA, 细胞凋亡, 细胞周期

Abstract: Objective To explore the effect of Tanshinone ⅡA on cell proliferation,apoptosis and cell cycle of fibroblasts derived from keloid.Methods Fibroblasts derived from keloid were cultured with different concentration of Tanshinone ⅡA(0 μg/mL,50 μg/mL,100 μg/mL and 200 μg/mL) in vitro.CCK-8 was used to detect cell proliferation,and flow cytometry was used to analyze cell apoptosis,cell cycle at the different time.Results Cell proliferation of fibroblasts derived from keloid was decreased,apoptosis at the early phase was increased,and cell cycle in G0/G1 phase was arrested by different concentration of Tanshinone ⅡA with different intervention time(P<0.001),especially in 200 μg/mL group.Variance analysis showed that those differences have statistical significance.Conclusion Tanshinon ⅡA can suppress cell proliferation,induce cell apoptosis and arrest cell cycle of fibroblasts derived from keloid.The effect of Tanshinone ⅡA was affected by different concentration and different intervention time.

Key words: Keloid, Fibroblast, Tanshinone ⅡA, Apoptosis, Cell cycle

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