组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2014, Vol. 10 ›› Issue (5): 259-262.doi: 10.3969/j.issn.1673-0364.2014.05.006

• 论著 • 上一篇    下一篇

柠檬酸酸蚀后再植牙根表面的脱矿研究

朱文婷，汪俊，岑莲   

  1. 上海交通大学医学院附属第九人民医院儿童口腔科,上海口腔医学重点实验室；组织工程国家工程研究中心；华东理工大学化工学院
  • 发布日期:2020-07-23

The Texture of Replanted Root Surface and Early Cell Colonization After Citric Acid Demineralization

ZHU Wenting,WANG Jun,CEN Lian   

  1. Department of Pediatric Dentistry, Shanghai Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology;National Tissue Engineering Center of China;School of Chemical Engineering, East China University of Science and Technology;
  • Published:2020-07-23
  • Contact: 国家重点基础研究发展计划（“973”项目，2010cb944804）；上海市科学技术委员会医学引导项目（124119b0101）

摘要： 目的研究不同脱矿方法酸蚀牙根后,根面形貌的变化及胶原纤维暴露面积,探索牙根再植的最佳存活条件。方法收集离体狗牙,制备5 mm×5 mm大小的根片,随机分为7组:A组未经酸蚀处理,即空白对照组;B组采用30%柠檬酸浸泡处理1 h;其余5组使用浸有30%柠檬酸溶液的小棉球擦拭牙面,时间分别为3 min、5 min、10 min、20 min和30 min,设为C-3、C-5、C-10、C-20、C-30组。处理后样本扫描电镜观察根面微观形貌。热重分析仪SDT检测酸蚀处理对牙根无机物损耗的影响。在根面接种骨髓间充质细胞,观察细胞早期附着及增殖情况。结果未处理的根面可见明显玷污层,经柠檬酸浸泡1 h后根表面光滑,未见有暴露的胶原纤维。而使用擦拭方法处理的根面不仅能去除玷污层,而且能暴露胶原纤维,且随擦拭时间的变化暴露的胶原量也有明显差异。C-5组根面暴露的胶原面积高于C-3组,而处理时间超过10 min的根面可以发现牙骨质的大面积脱矿,牙本质小管暴露,且胶原纤维也随着牙骨质的剥脱而破坏。此外,接种在酸蚀根面的细胞附着形态较好,增殖活性强于未处理根面。结论柠檬酸根面擦拭处理5 min为理想的再植牙根表面处理方式。

关键词: 柠檬酸, 根面处理, 细胞定植

Abstract: Objective To investigate the texture of root surface and collagen fiber exposure after etching with different modes of citric acid, and to explore the best condition for root replantation. Methods Root pieces （5 mm×5 mm） were prepared from molars of beagle dog and randomly divided into seven groups. Group A was treated as negative control, and root pieces in group B were soaking in 30% citric acid solution for 1 hour. The root surfaces of remaining five groups were rubbed with cotton pellets soaked in the 30% citric acid solution for 3 min, 5 min, 10 min, 20 rain and 30 min separately. Scanning electron microscopy was used to observe the presence of smear layer and the area of exposed collagen fibers. Inorganic loss after acid etching was detected by thermo gravimetric analyzer. BMSCs were seeded and cultured for 24 hour on the surface of citric acid-etched root. The condition of cell proliferation was tested by Cell Counting Kit-8. Results Smear layer was observed in group A. The root surface in group B was smooth, without any exposed collagen fibers. Collagen fibers exposure was observed in group C with no smear layer. The exposed amount of collagen was different according to the different processing time. The exposed amount of collagen in C-5 group was higher than in C-3 group, and the processing time of more than 10 minutes were appeared to remove not only the mineral component but also the collagenous matrix. Cell adhesion and cell proliferation in etched group were better than in control group. Conclusion The root surfacerubbed with cotton pellets soaked in the 30% citric acid solution for 5 min seems to be an ideal root surface conditioning method.

Key words: Citric acid, Root surface conditioning, Cell colonization

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