组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2008, Vol. 4 ›› Issue (6): 305-307.doi: 10.3969/j.issn.1673-0364.2008.06.002

• 论著 • 上一篇    下一篇

不同比例的PLA-PGA支架对软骨细胞复合的影响

张路，李琼，周广东，邓丹，刘伟，曹谊林   

  1. 上海交通大学医学院附属第九人民医院整复外科;上海市组织工程重点实验室;
  • 发布日期:2020-07-23

The Impact of Different Proportion of PLA Embeded PLA-PGA Ccaffold on Swine Chondrocytes

ZHANG Lu,LIQiong,ZHOU Guangdong,DENG Dan,LIU Wei,CAO Yilin   

  1. Department of Plastic and Reconstructive Surgery;Shanghai Ninth People s Hospital;Shanghai Jiaotong University School of Medicine;Shanghai 200011;China.;
  • Published:2020-07-23
  • Contact: 国家高技术发展计划(863)重大专项(2002AA205021);;国家重点基础研究发展计划(973)(2005CB522702);;国家自然科学基金项目(30300353);;上海市重点实验室基金(06DZ22029)。

摘要： 目的研究经聚乳酸(PLA)包埋后的不同比例PLA-聚羟基乙酸(PGA)组织工程支架对软骨细胞复合的影响及原因。方法分离培养小猪软骨细胞,并以5×107cells/mL的密度接种在经PLA包埋的不同比例PGA-PLA支架材料上,4h后检测软骨细胞在支架材料上的流失率,24h后检测软骨细胞在支架材料上的粘附率,并对支架材料进行电镜扫描,分析原因。结果随着支架材料中PLA比例的提高,软骨细胞的接种流失率上升,支架材料的接种负荷下降,24h时软骨细胞粘附率无明显差异。扫描电镜证实其原因。结论PLA包埋的PGA支架材料会降低对软骨细胞的接种负荷,但细胞粘附率无变化。

关键词: 组织工程, 聚羟基乙酸, 聚乳酸, 粘附率, 软骨细胞

Abstract: Objective To investigate the impact of different proportion of PLA embeded PLA-PGA scaffold on swine chondrocytes. Methods Swine chondrocytes were harvested and cultured, and seeded onto the PGA-PLA scaffold of different groups at the concentration of 5×107 cells/mL. The cell run off rate was examined after 4 hours, and the cell adherence rate was examined after 24 hours. In addition, we did the PGA-PLA scaffold was examined by scanning electron microscope. Results With the rise of the proportion of PLA, th...

Key words: Tissue engineering, PGA, PLA, Cell adherence rate, Chondrocyte

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