组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2023, Vol. 19 ›› Issue (3): 229-.

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基于体表压力传感器监测的压力疗法对增生性瘢痕的疗效观察

  

  • 出版日期:2023-06-01 发布日期:2023-07-05

 Effect of pressure therapy on hypertrophic scar based on body surface pressure sensor monitoring

  • Online:2023-06-01 Published:2023-07-05

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摘要：

目的　研究锶 -β- 磷酸三钙（Sr-β-TCP）对兔脂肪干细胞（Rabbit adipose-derived stem cells，rADSC）生
物学活性和成骨分化的影响。方法　取新西兰大白兔肩胛间区皮下脂肪，分离培养 rADSCs，选取第 3 代 rADSCs 进
行成骨诱导。制备 β-TCP 材料和 Sr-β-TCP 材料，将 β-TCP 材料和 Sr-β-TCP 分别与成骨诱导的 rADSCs 共培养，
分为 1% Sr-β-TCP 组、3% Sr-β-TCP 组、5% Sr-β-TCP 组、β-TCP 组、对照组（成骨诱导培养液）。MTT 比色法检
测 rADSCs 增殖情况，检测碱性磷酸酶（Alkaline phosphatase，ALP）活性及钙离子含量，蛋白质免疫印迹（Western
blot）检测 rADSCs 成骨相关蛋白表达。结果　与对照组和 3% Sr-β-TCP 组相比，β-TCP 组、1% 及 5%Sr-β-TCP 组
d1、d3、d6、d8 的 OD 值均下降（P<0.05）；d3、d6、d8，5%Sr-β-TCP 组 OD 值均低于 β-TCP 组、1%Sr-β-TCP 组（P<0.05）。
随着时间延长，各组光密度（OD）值均升高（P<0.05）。与对照组和 β-TCP 组相比，1%、3% Sr-β-TCP 组钙离子含量，
ALP 活性，Notch3、pFAK、pERK1/2、RhoA 表达升高，5% Sr-β-TCP 组钙离子含量，ALP 活性，Notch3、pFAK、
pERK1/2、RhoA 表达降低（P<0.05）；3% Sr-β-TCP 组钙离子含量，ALP 活性，Notch3、pFAK、pERK1/2、RhoA 表
达高于 1%、5% Sr-β-TCP 组（P<0.05）。结论　3% Sr-β-TCP 材料能提高 rADSCs 生物学活性以及成骨分化能力，
可能与 Notch3/FAK/ERK1/2 信号通路有关。

关键词:

Abstract:

Objective　To investigate the effects of strontium-β-tricalcium phosphate (SR-β-TCP) on the biological activ
ity and osteogenic differentiation of rabbit adipose derived stem cells (ADSCs). Methods　rADSCs were isolated and cultured
from subcutaneous fat of interscapular region of New Zealand white rabbits, and the third generation rADSCs were selected for
osteogenic induction. β-TCP materials and Sr-β-TCP materials were prepared, and the β-TCP materials and Sr-β-TCP materi
als were co-cultured with osteogenic induced rADSCs, respectively, and divided into 1% Sr-β-TCP group, 3% Sr-β-TCP group,
5% Sr-β-TCP group, β-TCP group and control group (osteogenic induction medium). The proliferation of rADSCs was detected
by MTT colorimetric assay, and the activity of Alkaline phosphatase (ALP) and calcium ion content were detected. The osteogen
ic related proteins of rADSCs were detected by Western blot (WB). Results　Compared with the control group and 3% Sr-β-
TCP group, the optical density (OD) value of β-TCP group, 1% and 5% Sr-β-TCP group decreased on the 1st, 3rd, 6th and 8th
day (P<0.05). The OD value of 5% Sr-β-TCP group was lower than that of β-TCP group and 1% Sr-β-TCP group on the 3rd,
6th and 8th day (P<0.05). OD values of all groups were increased with the extension of time (P<0.05). Compared with the control
group and β-TCP group, the calcium ion content, ALP activity, Notch3, pFAK, pERK1/2 and RhoA expression of 1% and 3% 
Sr-β- TCP groups were increased, while the calcium ion content, ALP activity, Notch3, pFAK, pERK1/2 and RhoA expression of 5%
Sr-β-TCP group were decreased (P<0.05). The calcium ion content, ALP activity, Notch3, pFAK, pERK1/2 and RhoA expression
of 3% Sr-β-TCP group were higher than those of 1% and 5% Sr-β-TCP group (P<0.05). Conclusion　3% Sr-β-TCP can improve
the biological activity and osteogenic differentiation of rADSCs, which may be related to Notch3/FAK/ERK1/2 signaling pathway.

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