组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2023, Vol. 19 ›› Issue (3): 275-.

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基质细胞衍生因子 -1α 联合富血小板纤维蛋白在种植牙骨组织再生中的应用研究

  

  • 出版日期:2023-06-01 发布日期:2023-07-05

Application of stromal cell-derived factor-1 α combined with platelet-rich fibrin in bone regeneration of dental implants

  • Online:2023-06-01 Published:2023-07-05

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摘要：

目的　探讨基质细胞衍生因子 -1α 联合富血小板纤维蛋白，在促进种植牙牙槽骨组织再生过程中的作用。
方法　以 2019 年 6 月至 2020 年 12 月收治的 120 例种植牙患者为研究对象，根据随机数字表法分为实验组（n=60）
和对照组（n=60）。对照组采用富血小板纤维蛋白 + 人工骨粉，实验组采用基质细胞衍生因子 -1α+ 富血小板纤维
蛋白 + 人工骨粉。对比两组患者的骨密度、骨厚度、牙槽骨唇舌向宽度、牙龈健康情况、种植牙成功率、完全愈合
率、局部感染率、种植体稳定系数（ISQ）、探诊深度（PD）、龈沟出血指数（SBI）；术后 7 ｄ，酶联免疫吸附试验
和 Western blot 检测龈沟液和骨屑中血小板衍生生长因子（PDGF）、转化生长因子 -β（TGF-β）、骨形态发生蛋白 2
（BMP-2）表达水平。结果　术后 7 d，实验组 PDGF、TGF-β、BMP-2 蛋白表达水平高于对照组（P<0.05）。术后 6 个月，
实验组骨密度、骨厚度、牙槽骨唇舌向宽度、种植体稳定系数高于对照组（P<0.05），探诊深度和龈沟出血指数低于
对照组（P<0.05）；两组患者种植牙成功率、完全愈合率和局部感染率对比，差异无统计学意义（P>0.05）。结论
基质细胞衍生因子 -1α 联合富血小板纤维蛋白可以促进种植牙患者牙槽骨组织再生修复，相关作用机制可能与上调
PDGF、TGF-β 和 BMP-2 表达有关。

关键词:

Abstract:

Objective　To investigate the effect of stromal cell derived factor-1 α combined with platelet-rich fibrin on alve
olar bone regeneration after dental implantation. Methods　A total of 120 dental implant patients were selected from June 2019
to December 2020. According to random number table method, patients were divided into experimental group (n=60) and con
trol group (n=60). The control group was treated with platelet-rich fibrin + artificial bone meal, and the experimental group was
treated with stromal cell-derived factor-1 α + platelet-rich fibrin + artificial bone meal. Bone mineral density, bone thickness,
alveolar bone lip and tongue width, gingival health, implant success rate, complete healing rate, local infection rate, implant sta
bility coefficient (ISQ), probing depth (PD) and gingival crevicular bleeding index (SBI) were compared between the two groups.
Enzyme-linked immunosorbent assay and Western blot was used to detect the expression levels of platelet-derived growth factor
(PDGF), transforming growth factor (TGF-β) and bone morphogenetic protein 2 (BMP-2) in gingival crevicular fluid and bone
chips 7 days after operation. Results　The expression levels of PDGF, TGF-β and BMP-2 protein in the experimental group
were higher than those in the control group on the 7th day after surgery (P<0.05). The bone density, bone thickness, alveolar bone
lip-lingual width and implant stability coefficient in the experimental group were higher than those in the control group at the 6th
month after operation (P<0.05). The probing depth and gingival crevicular bleeding index were lower than those in the control 
group (P<0.05). There was no significant difference in the implant success rate, complete healing rate and local infection rate be
tween the two groups 6 months after surgery (P>0.05). Conclusion　Stromal cell derived factor-1 α combined with platelet-rich
fibrin can promote alveolar bone regeneration and repair in patients with dental implants, and the relevant mechanism may be
related to the up-regulation of PDGF, TGF-β and BMP-2 expression.

Key words:

mineral density