关键词: 神经嵴, 　谱系追踪, 　细胞迁移, 　嵌合体

Abstract: The neural crest is a migratory， multipotent stem cell population during vertebrate embryogenesis， and lineage tracing is the focus of its research. Over the 150 years since its first discovery， understanding of the neural crest has gone through a transition from morphological observations to single-cell level. In this paper， important approaches applied to study the neural crest were reviewed including radioisotope labeling， chimeras， vital dye labeling， in vitro clonal analysis， conditional genetic recombination and single-cell sequencing.

Key words: Neural crest, 　Lineage tracing, 　Cell migration, 　Chimeras